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10.1016/j.molcel.2021.07.027 http://scihub22266oqcxt.onion/10.1016/j.molcel.2021.07.027 34390675!ä!34390675 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Cell 2021 ; 81 (17): 3650-3658.e5 Nephropedia Template TP {{tp|p=34390675|t=2021. CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays |pdf=|usr=}}{{34390675}} gab.com Text CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays JO: Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=34390675 #FOAvip CRISPR-inspired systems have been extensively developed for applications in genome editing and nucleic acid detection. Here, we introduce a CRISPR-based peptide display technology to facilitate customized, high-throughput in vitro protein interaction studies. We show that bespoke peptide libraries fused to catalytically inactive Cas9 (dCas9) and barcoded with unique single guide RNA (sgRNA) molecules self-assemble from a single mixed pool to programmable positions on a DNA microarray surface for rapid, multiplexed binding assays. We develop dCas9-displayed saturation mutagenesis libraries to characterize antibody-epitope binding for a commercial anti-FLAG monoclonal antibody and human serum antibodies. We also show that our platform can be used for viral epitope mapping and exhibits promise as a multiplexed diagnostics tool. Our CRISPR-based peptide display platform and the principles of complex library self-assembly using dCas9 could be adapted for rapid interrogation of varied customized protein libraries or biological materials assembly using DNA scaffolding. Twit Text FOAVip CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays ????Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=34390675 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34390675 #FOAvip English Wikipedia <ref>{{Cite pmid|34390675}}</ref> CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays #MMPMID34390675 Barber KW; Shrock E; Elledge SJ Mol Cell 2021[Sep]; 81 (17): 3650-3658.e5 PMID34390675show ga CRISPR-inspired systems have been extensively developed for applications in genome editing and nucleic acid detection. Here, we introduce a CRISPR-based peptide display technology to facilitate customized, high-throughput in vitro protein interaction studies. We show that bespoke peptide libraries fused to catalytically inactive Cas9 (dCas9) and barcoded with unique single guide RNA (sgRNA) molecules self-assemble from a single mixed pool to programmable positions on a DNA microarray surface for rapid, multiplexed binding assays. We develop dCas9-displayed saturation mutagenesis libraries to characterize antibody-epitope binding for a commercial anti-FLAG monoclonal antibody and human serum antibodies. We also show that our platform can be used for viral epitope mapping and exhibits promise as a multiplexed diagnostics tool. Our CRISPR-based peptide display platform and the principles of complex library self-assembly using dCas9 could be adapted for rapid interrogation of varied customized protein libraries or biological materials assembly using DNA scaffolding. |*Peptide Library[MESH] |CRISPR-Cas Systems/genetics/immunology[MESH] |Epitopes/*genetics/immunology[MESH] |Gene Editing/*methods[MESH] |Humans[MESH] |Mutagenesis/genetics[MESH] |Protein Binding/genetics/immunology[MESH]CRISPR-based peptide library display and programmable microarray self-(epmc).pdf DeepDyve Pubget Overpricing