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10.1515/cclm-2021-0483

http://scihub22266oqcxt.onion/10.1515/cclm-2021-0483
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34388325!ä!34388325

suck abstract from ncbi


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pmid34388325      Clin+Chem+Lab+Med 2021 ; 59 (12): 1998-2002
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  • Alternative detection of SARS-CoV-2 RNA by a new assay based on mass spectrometry #MMPMID34388325
  • Stelzl E; Kessler HH; Mustafa HG; Mustafa ME; Santner BI; Seier J; La Torre M; Haushofer AC
  • Clin Chem Lab Med 2021[Nov]; 59 (12): 1998-2002 PMID34388325show ga
  • OBJECTIVES: Accurate detection of SARS-CoV-2 RNA is essential to stopping the spread of SARS-CoV-2. The aim of this study was to evaluate the performance of the recently introduced MassARRAY((R)) SARS-CoV-2 Panel and to compare it to the cobas((R)) SARS-CoV-2 Test. METHODS: The MassARRAY((R)) SARS-CoV-2 Panel consists of five assays targeting different sequences of the SARS-CoV-2 genome. Accuracy was determined using national and international proficiency panels including 27 samples. For clinical evaluation, 101 residual clinical samples were analyzed and results compared. Samples had been tested for SARS-CoV-2 RNA with the cobas((R)) SARS-CoV-2 Test. RESULTS: When accuracy was tested with the MassARRAY((R)) SARS-CoV-2 Panel, 25 of 27 (92.6%) samples revealed correct results. When clinical samples were analyzed with the MassARRAY((R)) SARS-CoV-2 Panel and compared to the cobas((R)) SARS-CoV-2 Test, 100 samples showed concordant results. One sample was found to be inconclusive with the MassARRAY((R)) SARS-CoV-2 Panel. When time-to-results were compared, the new assay showed longer total and hands-on times. CONCLUSIONS: The MassARRAY((R)) SARS-CoV-2 Panel showed a good performance and proved to be suitable for use in the routine diagnostic laboratory. Especially during phases of shortage of reagents and/or disposables, the new test system appears as beneficial alternative to standard assays used for detection of SARS-CoV-2 RNA.
  • |COVID-19 Testing/*methods[MESH]
  • |COVID-19/*diagnosis/virology[MESH]
  • |Humans[MESH]
  • |Mass Spectrometry[MESH]
  • |Nasopharynx/virology[MESH]
  • |RNA, Viral/*analysis/metabolism[MESH]
  • |Reagent Kits, Diagnostic[MESH]
  • |Real-Time Polymerase Chain Reaction[MESH]
  • |Reproducibility of Results[MESH]


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