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10.3389/fimmu.2021.719037 http://scihub22266oqcxt.onion/10.3389/fimmu.2021.719037 34381460!8351461!34381460     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Front+Immunol 2021 ; 12 (ä): 719037 Nephropedia Template TP {{tp|p=34381460|t=2021. Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies |pdf=|usr=}}{{34381460}} gab.com Text Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies JO: Front Immunol http://www.kidney.de/pget.php?&tw=1&pmid=34381460 #FOAvip The COVID-19 pandemic, caused by the coronavirus SARS-CoV-2, is the most severe public health event of the twenty-first century. While effective vaccines against SARS-CoV-2 have been developed, there remains an urgent need for diagnostics to quickly and accurately detect infections. Antigen tests, particularly those that detect the abundant SARS-CoV-2 Nucleocapsid protein, are a proven method for detecting active SARS-CoV-2 infections. Here we report high-resolution crystal structures of three llama-derived single-domain antibodies that bind the SARS-CoV-2 Nucleocapsid protein with high affinity. Each antibody recognizes a specific folded domain of the protein, with two antibodies recognizing the N-terminal RNA binding domain and one recognizing the C-terminal dimerization domain. The two antibodies that recognize the RNA binding domain affect both RNA binding affinity and RNA-mediated phase separation of the Nucleocapsid protein. All three antibodies recognize highly conserved surfaces on the Nucleocapsid protein, suggesting that they could be used to develop affordable diagnostic tests to detect all circulating SARS-CoV-2 variants. Twit Text FOAVip Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies ????Front Immunol http://www.kidney.de/pget.php?&tw=1&pmid=34381460 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34381460 #FOAvip English Wikipedia <ref>{{Cite pmid|34381460}}</ref> Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies #MMPMID34381460 Ye Q; Lu S; Corbett KD Front Immunol 2021[]; 12 (ä): 719037 PMID34381460show ga The COVID-19 pandemic, caused by the coronavirus SARS-CoV-2, is the most severe public health event of the twenty-first century. While effective vaccines against SARS-CoV-2 have been developed, there remains an urgent need for diagnostics to quickly and accurately detect infections. Antigen tests, particularly those that detect the abundant SARS-CoV-2 Nucleocapsid protein, are a proven method for detecting active SARS-CoV-2 infections. Here we report high-resolution crystal structures of three llama-derived single-domain antibodies that bind the SARS-CoV-2 Nucleocapsid protein with high affinity. Each antibody recognizes a specific folded domain of the protein, with two antibodies recognizing the N-terminal RNA binding domain and one recognizing the C-terminal dimerization domain. The two antibodies that recognize the RNA binding domain affect both RNA binding affinity and RNA-mediated phase separation of the Nucleocapsid protein. All three antibodies recognize highly conserved surfaces on the Nucleocapsid protein, suggesting that they could be used to develop affordable diagnostic tests to detect all circulating SARS-CoV-2 variants. |COVID-19[MESH] |COVID-19 Serological Testing/*methods[MESH] |Coronavirus Infections/diagnosis/immunology[MESH] |Coronavirus Nucleocapsid Proteins/*immunology/*ultrastructure[MESH] |Crystallography, X-Ray[MESH] |Humans[MESH] |Protein Domains[MESH] |SARS-CoV-2/immunology[MESH]Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Si(epmc).pdf DeepDyve Pubget Overpricing