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10.1016/j.celrep.2021.109530 http://scihub22266oqcxt.onion/10.1016/j.celrep.2021.109530 34380018!8316065!34380018     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34380018&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Rep 2021 ; 36 (7): 109530 Nephropedia Template TP {{tp|p=34380018|t=2021. No evidence of human genome integration of SARS-CoV-2 found by long-read DNA sequencing |pdf=|usr=}}{{34380018}} gab.com Text No evidence of human genome integration of SARS-CoV-2 found by long-read DNA sequencing JO: Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=34380018 #FOAvip A recent study proposed that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) hijacks the LINE-1 (L1) retrotransposition machinery to integrate into the DNA of infected cells. If confirmed, this finding could have significant clinical implications. Here, we apply deep (>50x) long-read Oxford Nanopore Technologies (ONT) sequencing to HEK293T cells infected with SARS-CoV-2 and do not find the virus integrated into the genome. By examining ONT data from separate HEK293T cultivars, we completely resolve 78 L1 insertions arising in vitro in the absence of L1 overexpression systems. ONT sequencing applied to hepatitis B virus (HBV)-positive liver cancer tissues located a single HBV insertion. These experiments demonstrate reliable resolution of retrotransposon and exogenous virus insertions by ONT sequencing. That we find no evidence of SARS-CoV-2 integration suggests that such events are, at most, extremely rare in vivo and therefore are unlikely to drive oncogenesis or explain post-recovery detection of the virus. Twit Text FOAVip No evidence of human genome integration of SARS-CoV-2 found by long-read DNA sequencing ????Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=34380018 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34380018 #FOAvip English Wikipedia <ref>{{Cite pmid|34380018}}</ref> No evidence of human genome integration of SARS-CoV-2 found by long-read DNA sequencing #MMPMID34380018 Smits N; Rasmussen J; Bodea GO; Amarilla AA; Gerdes P; Sanchez-Luque FJ; Ajjikuttira P; Modhiran N; Liang B; Faivre J; Deveson IW; Khromykh AA; Watterson D; Ewing AD; Faulkner GJ Cell Rep 2021[Aug]; 36 (7): 109530 PMID34380018show ga A recent study proposed that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) hijacks the LINE-1 (L1) retrotransposition machinery to integrate into the DNA of infected cells. If confirmed, this finding could have significant clinical implications. Here, we apply deep (>50x) long-read Oxford Nanopore Technologies (ONT) sequencing to HEK293T cells infected with SARS-CoV-2 and do not find the virus integrated into the genome. By examining ONT data from separate HEK293T cultivars, we completely resolve 78 L1 insertions arising in vitro in the absence of L1 overexpression systems. ONT sequencing applied to hepatitis B virus (HBV)-positive liver cancer tissues located a single HBV insertion. These experiments demonstrate reliable resolution of retrotransposon and exogenous virus insertions by ONT sequencing. That we find no evidence of SARS-CoV-2 integration suggests that such events are, at most, extremely rare in vivo and therefore are unlikely to drive oncogenesis or explain post-recovery detection of the virus. |*Genome, Human[MESH] |*Sequence Analysis, DNA[MESH] |*Virus Integration[MESH] |Aged[MESH] |Animals[MESH] |COVID-19/diagnosis/*virology[MESH] |Carcinoma, Hepatocellular/virology[MESH] |Chlorocebus aethiops[MESH] |DNA, Viral/*genetics[MESH] |HEK293 Cells[MESH] |Hepatitis B virus/genetics[MESH] |Host-Pathogen Interactions[MESH] |Humans[MESH] |Liver Neoplasms/virology[MESH] |Long Interspersed Nucleotide Elements[MESH] |Male[MESH] |Nanopore Sequencing[MESH] |SARS-CoV-2/*genetics[MESH]No evidence of human genome integration of SARS-CoV-2 found by long-re(epmc).pdf DeepDyve Pubget Overpricing