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10.1002/bab.2239 http://scihub22266oqcxt.onion/10.1002/bab.2239 34378814!8427135!34378814     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biotechnol+Appl+Biochem 2022 ; 69 (4): 1696-1711 Nephropedia Template TP {{tp|p=34378814|t=2022. MicroRNA of N-region from SARS-CoV-2: Potential sensing components for biosensor development |pdf=|usr=}}{{34378814}} gab.com Text MicroRNA of N-region from SARS-CoV-2: Potential sensing components for biosensor development JO: Biotechnol Appl Biochem http://www.kidney.de/pget.php?&tw=1&pmid=34378814 #FOAvip An oligonucleotide DNA probe has been developed for the application in the DNA electrochemical biosensor for the early diagnosis of coronavirus disease (COVID-19). Here, the virus microRNA from the N-gene of severe acute respiratory syndrome-2 (SARS-CoV-2) was used for the first time as a specific target for detecting the virus and became a framework for developing the complementary DNA probe. The sequence analysis of the virus microRNA was carried out using bioinformatics tools including basic local alignment search tools, multiple sequence alignment from CLUSTLW, microRNA database (miRbase), microRNA target database, and gene analysis. Cross-validation of distinct strains of coronavirus and human microRNA sequences was completed to validate the percentage of identical and consent regions. The percent identity parameter from the bioinformatics tools revealed the virus microRNAs' sequence has a 100% match with the genome of SARS-CoV-2 compared with other coronavirus strains, hence improving the selectivity of the complementary DNA probe. The 30 mer with 53.0% GC content of complementary DNA probe 5' GCC TGA GTT GAG TCA GCA CTG CTC ATG GAT 3' was designed and could be used as a bioreceptor for the biosensor development in the clinical and environmental diagnosis of COVID-19. Twit Text FOAVip MicroRNA of N-region from SARS-CoV-2: Potential sensing components for biosensor development ????Biotechnol Appl Biochem http://www.kidney.de/pget.php?&tw=1&pmid=34378814 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34378814 #FOAvip English Wikipedia <ref>{{Cite pmid|34378814}}</ref> MicroRNA of N-region from SARS-CoV-2: Potential sensing components for biosensor development #MMPMID34378814 Halim FS; Parmin NA; Hashim U; Gopinath SCB; Dahalan FA; Zakaria II; Ang WC; Jaapar NF Biotechnol Appl Biochem 2022[Aug]; 69 (4): 1696-1711 PMID34378814show ga An oligonucleotide DNA probe has been developed for the application in the DNA electrochemical biosensor for the early diagnosis of coronavirus disease (COVID-19). Here, the virus microRNA from the N-gene of severe acute respiratory syndrome-2 (SARS-CoV-2) was used for the first time as a specific target for detecting the virus and became a framework for developing the complementary DNA probe. The sequence analysis of the virus microRNA was carried out using bioinformatics tools including basic local alignment search tools, multiple sequence alignment from CLUSTLW, microRNA database (miRbase), microRNA target database, and gene analysis. Cross-validation of distinct strains of coronavirus and human microRNA sequences was completed to validate the percentage of identical and consent regions. The percent identity parameter from the bioinformatics tools revealed the virus microRNAs' sequence has a 100% match with the genome of SARS-CoV-2 compared with other coronavirus strains, hence improving the selectivity of the complementary DNA probe. The 30 mer with 53.0% GC content of complementary DNA probe 5' GCC TGA GTT GAG TCA GCA CTG CTC ATG GAT 3' was designed and could be used as a bioreceptor for the biosensor development in the clinical and environmental diagnosis of COVID-19. |*Biosensing Techniques[MESH] |*COVID-19/diagnosis[MESH] |*MicroRNAs/genetics[MESH] |DNA Probes[MESH] |DNA, Complementary[MESH] |Genome, Viral[MESH] |Humans[MESH]MicroRNA of N-region from SARS-CoV-2: Potential sensing components for(epmc).pdf DeepDyve Pubget Overpricing