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10.1038/s41421-021-00300-2 http://scihub22266oqcxt.onion/10.1038/s41421-021-00300-2 34373443!8352953!34373443     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Discov 2021 ; 7 (1): 64 Nephropedia Template TP {{tp|p=34373443|t=2021. Integrated single-cell analysis revealed immune dynamics during Ad5-nCoV immunization |pdf=|usr=}}{{34373443}} gab.com Text Integrated single-cell analysis revealed immune dynamics during Ad5-nCoV immunization JO: Cell Discov http://www.kidney.de/pget.php?&tw=1&pmid=34373443 #FOAvip Coronavirus disease 2019 (COVID-19), driven by SARS-CoV-2, is a severe infectious disease that has become a global health threat. Vaccines are among the most effective public health tools for combating COVID-19. Immune status is critical for evaluating the safety and response to the vaccine, however, the evolution of the immune response during immunization remains poorly understood. Single-cell RNA sequencing (scRNA-seq) represents a powerful tool for dissecting multicellular behavior and discovering therapeutic antibodies. Herein, by performing scRNA/V(D)J-seq on peripheral blood mononuclear cells from four COVID-19 vaccine trial participants longitudinally during immunization, we revealed enhanced cellular immunity with concerted and cell type-specific IFN responses as well as boosted humoral immunity with SARS-CoV-2-specific antibodies. Based on the CDR3 sequence and germline enrichment, we were able to identify several potential binding antibodies. We synthesized, expressed and tested 21 clones from the identified lineages. Among them, one monoclonal antibody (P3V6-1) exhibited relatively high affinity with the extracellular domain of Spike protein, which might be a promising therapeutic reagent for COVID-19. Overall, our findings provide insights for assessing vaccine through the novel scRNA/V(D)J-seq approach, which might facilitate the development of more potent, durable and safe prophylactic vaccines. Twit Text FOAVip Integrated single-cell analysis revealed immune dynamics during Ad5-nCoV immunization ????Cell Discov http://www.kidney.de/pget.php?&tw=1&pmid=34373443 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34373443 #FOAvip English Wikipedia <ref>{{Cite pmid|34373443}}</ref> Integrated single-cell analysis revealed immune dynamics during Ad5-nCoV immunization #MMPMID34373443 Cao Q; Wu S; Xiao C; Chen S; Chi X; Cui X; Tang H; Su W; Zheng Y; Zhong J; Li Z; Li F; Chen H; Hou L; Wang H; Wen W Cell Discov 2021[Aug]; 7 (1): 64 PMID34373443show ga Coronavirus disease 2019 (COVID-19), driven by SARS-CoV-2, is a severe infectious disease that has become a global health threat. Vaccines are among the most effective public health tools for combating COVID-19. Immune status is critical for evaluating the safety and response to the vaccine, however, the evolution of the immune response during immunization remains poorly understood. Single-cell RNA sequencing (scRNA-seq) represents a powerful tool for dissecting multicellular behavior and discovering therapeutic antibodies. Herein, by performing scRNA/V(D)J-seq on peripheral blood mononuclear cells from four COVID-19 vaccine trial participants longitudinally during immunization, we revealed enhanced cellular immunity with concerted and cell type-specific IFN responses as well as boosted humoral immunity with SARS-CoV-2-specific antibodies. Based on the CDR3 sequence and germline enrichment, we were able to identify several potential binding antibodies. We synthesized, expressed and tested 21 clones from the identified lineages. Among them, one monoclonal antibody (P3V6-1) exhibited relatively high affinity with the extracellular domain of Spike protein, which might be a promising therapeutic reagent for COVID-19. Overall, our findings provide insights for assessing vaccine through the novel scRNA/V(D)J-seq approach, which might facilitate the development of more potent, durable and safe prophylactic vaccines. äIntegrated single-cell analysis revealed immune dynamics during Ad5-nC(epmc).pdf DeepDyve Pubget Overpricing