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10.1017/ice.2021.369

http://scihub22266oqcxt.onion/10.1017/ice.2021.369
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suck abstract from ncbi


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pmid34369325      Infect+Control+Hosp+Epidemiol 2022 ; 43 (9): 1179-1183
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  • Impact of coronavirus disease 2019 (COVID-19) pre-test probability on positive predictive value of high cycle threshold severe acute respiratory coronavirus virus 2 (SARS-CoV-2) real-time reverse transcription polymerase chain reaction (RT-PCR) test results #MMPMID34369325
  • Gubbay JB; Rilkoff H; Kristjanson HL; Forbes JD; Murti M; Eshaghi A; Broukhanski G; Corbeil A; Fittipaldi N; Hopkins JP; Kristjanson E; Kus JV; Macdonald L; Majury A; Mallo GV; Mazzulli T; Melano RG; Olsha R; Perusini SJ; Tran V; Allen VG; Patel SN
  • Infect Control Hosp Epidemiol 2022[Sep]; 43 (9): 1179-1183 PMID34369325show ga
  • OBJECTIVES: Performance characteristics of SARS-CoV-2 nucleic acid detection assays are understudied within contexts of low pre-test probability, including screening asymptomatic persons without epidemiological links to confirmed cases, or asymptomatic surveillance testing. SARS-CoV-2 detection without symptoms may represent presymptomatic or asymptomatic infection, resolved infection with persistent RNA shedding, or a false-positive test. This study assessed the positive predictive value of SARS-CoV-2 real-time reverse transcription polymerase chain reaction (rRT-PCR) assays by retesting positive specimens from 5 pre-test probability groups ranging from high to low with an alternate assay. METHODS: In total, 122 rRT-PCR positive specimens collected from unique patients between March and July 2020 were retested using a laboratory-developed nested RT-PCR assay targeting the RNA-dependent RNA polymerase (RdRp) gene followed by Sanger sequencing. RESULTS: Significantly fewer (15.6%) positive results in the lowest pre-test probability group (facilities with institution-wide screening having
  • |*COVID-19/diagnosis[MESH]
  • |*Nucleic Acids[MESH]
  • |COVID-19 Testing[MESH]
  • |Humans[MESH]
  • |Predictive Value of Tests[MESH]
  • |Probability[MESH]
  • |RNA[MESH]
  • |RNA-Dependent RNA Polymerase[MESH]
  • |Real-Time Polymerase Chain Reaction[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction[MESH]
  • |Reverse Transcription[MESH]


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