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10.1096/fj.202002742RR http://scihub22266oqcxt.onion/10.1096/fj.202002742RR 34365657!8441663!34365657     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       FASEB+J 2021 ; 35 (9): e21801 Nephropedia Template TP {{tp|p=34365657|t=2021. Spike protein of SARS-CoV-2 activates macrophages and contributes to induction of acute lung inflammation in male mice |pdf=|usr=}}{{34365657}} gab.com Text Spike protein of SARS-CoV-2 activates macrophages and contributes to induction of acute lung inflammation in male mice JO: FASEB J http://www.kidney.de/pget.php?&tw=1&pmid=34365657 #FOAvip The spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays a crucial role in mediating viral entry into host cells. However, whether it contributes to pulmonary hyperinflammation in patients with coronavirus disease 2019 is not well known. In this study, we developed a spike protein-pseudotyped (Spp) lentivirus with the proper tropism of the SARS-CoV-2 spike protein on the surface and determined the distribution of the Spp lentivirus in wild-type C57BL/6J male mice that received an intravenous injection of the virus. Lentiviruses with vesicular stomatitis virus glycoprotein (VSV-G) or with a deletion of the receptor-binding domain (RBD) in the spike protein [Spp (?RBD)] were used as controls. Two hours postinfection (hpi), there were 27-75 times more viral burden from Spp lentivirus in the lungs than in other organs; there were also about 3-5 times more viral burden from Spp lentivirus than from VSV-G lentivirus in the lungs, liver, kidney, and spleen. Deletion of RBD diminished viral loads in the lungs but not in the heart. Acute pneumonia was observed in animals 24 hpi. Spp lentivirus was mainly found in SPC(+) and LDLR(+) pneumocytes and macrophages in the lungs. IL6, IL10, CD80, and PPAR-gamma were quickly upregulated in response to infection in the lungs as well as in macrophage-like RAW264.7 cells. Furthermore, forced expression of the spike protein in RAW264.7 cells significantly increased the mRNA levels of the same panel of inflammatory factors. Our results demonstrated that the spike protein of SARS-CoV-2 confers the main point of viral entry into the lungs and can induce cellular pathology. Our data also indicate that an alternative ACE2-independent viral entry pathway may be recruited in the heart and aorta. Twit Text FOAVip Spike protein of SARS-CoV-2 activates macrophages and contributes to induction of acute lung inflammation in male mice ????FASEB J http://www.kidney.de/pget.php?&tw=1&pmid=34365657 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34365657 #FOAvip English Wikipedia <ref>{{Cite pmid|34365657}}</ref> Spike protein of SARS-CoV-2 activates macrophages and contributes to induction of acute lung inflammation in male mice #MMPMID34365657 Cao X; Tian Y; Nguyen V; Zhang Y; Gao C; Yin R; Carver W; Fan D; Albrecht H; Cui T; Tan W FASEB J 2021[Sep]; 35 (9): e21801 PMID34365657show ga The spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays a crucial role in mediating viral entry into host cells. However, whether it contributes to pulmonary hyperinflammation in patients with coronavirus disease 2019 is not well known. In this study, we developed a spike protein-pseudotyped (Spp) lentivirus with the proper tropism of the SARS-CoV-2 spike protein on the surface and determined the distribution of the Spp lentivirus in wild-type C57BL/6J male mice that received an intravenous injection of the virus. Lentiviruses with vesicular stomatitis virus glycoprotein (VSV-G) or with a deletion of the receptor-binding domain (RBD) in the spike protein [Spp (?RBD)] were used as controls. Two hours postinfection (hpi), there were 27-75 times more viral burden from Spp lentivirus in the lungs than in other organs; there were also about 3-5 times more viral burden from Spp lentivirus than from VSV-G lentivirus in the lungs, liver, kidney, and spleen. Deletion of RBD diminished viral loads in the lungs but not in the heart. Acute pneumonia was observed in animals 24 hpi. Spp lentivirus was mainly found in SPC(+) and LDLR(+) pneumocytes and macrophages in the lungs. IL6, IL10, CD80, and PPAR-gamma were quickly upregulated in response to infection in the lungs as well as in macrophage-like RAW264.7 cells. Furthermore, forced expression of the spike protein in RAW264.7 cells significantly increased the mRNA levels of the same panel of inflammatory factors. Our results demonstrated that the spike protein of SARS-CoV-2 confers the main point of viral entry into the lungs and can induce cellular pathology. Our data also indicate that an alternative ACE2-independent viral entry pathway may be recruited in the heart and aorta. |Acute Disease[MESH] |Alveolar Epithelial Cells/virology[MESH] |Animals[MESH] |B7-1 Antigen[MESH] |Cell Line[MESH] |Inflammation Mediators[MESH] |Interleukin-10[MESH] |Interleukin-6[MESH] |Lentivirus/genetics/isolation & purification/metabolism[MESH] |Lung/immunology/pathology/virology[MESH] |Macrophages/*immunology/virology[MESH] |Male[MESH] |Membrane Glycoproteins[MESH] |Mice[MESH] |Mice, Inbred C57BL[MESH] |PPAR gamma[MESH] |Pneumonia, Viral/*immunology/*pathology[MESH] |RAW 264.7 Cells[MESH] |Spike Glycoprotein, Coronavirus/chemistry/genetics/*immunology/metabolism[MESH]Spike protein of SARS-CoV-2 activates macrophages and contributes to i(epmc).pdf DeepDyve Pubget Overpricing