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10.3390/diagnostics11071241 http://scihub22266oqcxt.onion/10.3390/diagnostics11071241 34359323!8306735!34359323     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Diagnostics+(Basel) 2021 ; 11 (7): ä Nephropedia Template TP {{tp|p=34359323|t=2021. Limitation of Screening of Different Variants of SARS-CoV-2 by RT-PCR |pdf=|usr=}}{{34359323}} gab.com Text Limitation of Screening of Different Variants of SARS-CoV-2 by RT-PCR JO: Diagnostics (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=34359323 #FOAvip Since January 2021, the diffusion of the most propagated SARS-CoV-2 variants in France (UK variant 20I/501Y.V1 (lineage B.1.1.7), 20H/H501Y.V2 (lineage B.1.351) and 20J/H501Y.V3 (lineage P.1)) were urgently screened, needing a surveillance with an RT-PCR screening assay. In this study, we evaluated one RT-PCR kit for this screening (ID SARS-CoV-2/UK/SA Variant Triplex((R)), ID Solutions, Grabels, France) on 2207 nasopharyngeal samples that were positive for SARS-CoV-2. Using ID Solutions kit, 4.1% (92/2207) of samples were suspected to belonged to B.1.351 or P.1 variants. Next-generation sequencing that was performed on 67.4% (62/92) of these samples confirmed the presence of a B.1.351 variant in only 75.8% of the samples (47/62). Thirteen samples belonged to the UK variant (B.1.1.7), and two to A.27 with N501Y mutation. The thirteen with the UK variant presented one mutation in the S-gene, near the DeltaH69/DeltaV70 deletion (S71F or A67S), which impacted the detection of DeltaH69/DeltaV70 deletion. Using another screening kit (PKampVariantDetect SARS-CoV-2 RT-PCR combination 1 and 3((R)) PerkinElmer, Waltham, MA, USA) on the misidentified samples, we observed that the two mutations, S71F or A67S, did not impact the detection of the UK variant. In conclusion, this study highlights the limitations of the screening strategy based on the detection of few mutations/deletions as well as it not being able to follow the virus evolution. Twit Text FOAVip Limitation of Screening of Different Variants of SARS-CoV-2 by RT-PCR ????Diagnostics (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=34359323 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34359323 #FOAvip English Wikipedia <ref>{{Cite pmid|34359323}}</ref> Limitation of Screening of Different Variants of SARS-CoV-2 by RT-PCR #MMPMID34359323 Boudet A; Stephan R; Bravo S; Sasso M; Lavigne JP Diagnostics (Basel) 2021[Jul]; 11 (7): ä PMID34359323show ga Since January 2021, the diffusion of the most propagated SARS-CoV-2 variants in France (UK variant 20I/501Y.V1 (lineage B.1.1.7), 20H/H501Y.V2 (lineage B.1.351) and 20J/H501Y.V3 (lineage P.1)) were urgently screened, needing a surveillance with an RT-PCR screening assay. In this study, we evaluated one RT-PCR kit for this screening (ID SARS-CoV-2/UK/SA Variant Triplex((R)), ID Solutions, Grabels, France) on 2207 nasopharyngeal samples that were positive for SARS-CoV-2. Using ID Solutions kit, 4.1% (92/2207) of samples were suspected to belonged to B.1.351 or P.1 variants. Next-generation sequencing that was performed on 67.4% (62/92) of these samples confirmed the presence of a B.1.351 variant in only 75.8% of the samples (47/62). Thirteen samples belonged to the UK variant (B.1.1.7), and two to A.27 with N501Y mutation. The thirteen with the UK variant presented one mutation in the S-gene, near the DeltaH69/DeltaV70 deletion (S71F or A67S), which impacted the detection of DeltaH69/DeltaV70 deletion. Using another screening kit (PKampVariantDetect SARS-CoV-2 RT-PCR combination 1 and 3((R)) PerkinElmer, Waltham, MA, USA) on the misidentified samples, we observed that the two mutations, S71F or A67S, did not impact the detection of the UK variant. In conclusion, this study highlights the limitations of the screening strategy based on the detection of few mutations/deletions as well as it not being able to follow the virus evolution. äLimitation of Screening of Different Variants of SARS-CoV-2 by RT-PCR (epmc).pdf DeepDyve Pubget Overpricing