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10.3390/bios11070229

http://scihub22266oqcxt.onion/10.3390/bios11070229
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suck abstract from ncbi


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pmid34356700      Biosensors+(Basel) 2021 ; 11 (7): ä
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  • Comparative Study of In Situ Techniques to Enlarge Gold Nanoparticles for Highly Sensitive Lateral Flow Immunoassay of SARS-CoV-2 #MMPMID34356700
  • Panferov VG; Byzova NA; Biketov SF; Zherdev AV; Dzantiev BB
  • Biosensors (Basel) 2021[Jul]; 11 (7): ä PMID34356700show ga
  • Three techniques were compared for lowering the limit of detection (LOD) of the lateral flow immunoassay (LFIA) of the receptor-binding domain of severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) based on the post-assay in situ enlargement of Au nanoparticles (Au NPs) on a test strip. Silver enhancement (growth of a silver layer over Au NPs-Au@Ag NPs) and gold enhancement (growth of a gold layer over Au NPs) techniques and the novel technique of galvanic replacement of Ag by Au in Au@Ag NPs causing the formation of Au@Ag-Au NPs were performed. All the enhancements were performed on-site after completion of the conventional LFIA and maintained equipment-free assay. The assays demonstrated lowering of LODs in the following rows: 488 pg/mL (conventional LFIA with Au NPs), 61 pg/mL (silver enhancement), 8 pg/mL (galvanic replacement), and 1 pg/mL (gold enhancement). Using gold enhancement as the optimal technique, the maximal dilution of inactivated SARS-CoV-2-containing samples increased 500 times. The developed LFIA provided highly sensitive and rapid (8 min) point-of-need testing.
  • |Binding Sites[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Early Diagnosis[MESH]
  • |Gold/*chemistry[MESH]
  • |Humans[MESH]
  • |Immunoassay[MESH]
  • |Limit of Detection[MESH]
  • |Metal Nanoparticles[MESH]
  • |Point-of-Care Testing[MESH]
  • |SARS-CoV-2/*isolation & purification[MESH]
  • |Silver/*chemistry[MESH]


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