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10.1099/jmm.0.001402 http://scihub22266oqcxt.onion/10.1099/jmm.0.001402 34356000!8513627!34356000     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Microbiol 2021 ; 70 (8): ä Nephropedia Template TP {{tp|p=34356000|t=2021. Effect of multiple freeze-thaw cycles on the detection of anti-SARS-CoV-2 IgG antibodies |pdf=|usr=}}{{34356000}} gab.com Text Effect of multiple freeze-thaw cycles on the detection of anti-SARS-CoV-2 IgG antibodies JO: J Med Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=34356000 #FOAvip Several studies have investigated the effect of repeated freeze-thaw (F/T) cycles on RNA detection for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, no data are available regarding the effect of repeated F/T cycles on SARS-CoV-2 antibody detection in serum. We investigated the effect of multiple F/T cycles on anti-SARS-CoV-2 IgG detection using an ELISA test targeting the nucleocapsid antibodies. Ten positive and 1 negative SARS-CoV-2 IgG sera from 11 participants, in replicates of 5, were subjected to a total of 16 F/T cycles and stored at 4 degrees C until tested by ELISA. Statistical analysis was performed to test for F/T cycle effect. None of the 10 positive sera became negative after 16 F/T cycles. There was no significant difference in the OD average reading between the first and last F/T cycles, except for one serum with a minimal decline in the OD. The random effect linear regression of log (OD) on the number of cycles showed no significant trend, with a slope consistent with zero (B=-0.0001; 95 % CI -0.0008; 0.0006; P-value=0.781). These results suggest that multiple F/T cycles had no effect on the ability of the ELISA assay to detect SARS-CoV-2 IgG antibodies. Twit Text FOAVip Effect of multiple freeze-thaw cycles on the detection of anti-SARS-CoV-2 IgG antibodies ????J Med Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=34356000 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34356000 #FOAvip English Wikipedia <ref>{{Cite pmid|34356000}}</ref> Effect of multiple freeze-thaw cycles on the detection of anti-SARS-CoV-2 IgG antibodies #MMPMID34356000 Shurrab FM; Al-Sadeq DW; Amanullah F; Younes SN; Al-Jighefee H; Younes N; Dargham SR; Yassine HM; Abu Raddad LJ; Nasrallah GK J Med Microbiol 2021[Aug]; 70 (8): ä PMID34356000show ga Several studies have investigated the effect of repeated freeze-thaw (F/T) cycles on RNA detection for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, no data are available regarding the effect of repeated F/T cycles on SARS-CoV-2 antibody detection in serum. We investigated the effect of multiple F/T cycles on anti-SARS-CoV-2 IgG detection using an ELISA test targeting the nucleocapsid antibodies. Ten positive and 1 negative SARS-CoV-2 IgG sera from 11 participants, in replicates of 5, were subjected to a total of 16 F/T cycles and stored at 4 degrees C until tested by ELISA. Statistical analysis was performed to test for F/T cycle effect. None of the 10 positive sera became negative after 16 F/T cycles. There was no significant difference in the OD average reading between the first and last F/T cycles, except for one serum with a minimal decline in the OD. The random effect linear regression of log (OD) on the number of cycles showed no significant trend, with a slope consistent with zero (B=-0.0001; 95 % CI -0.0008; 0.0006; P-value=0.781). These results suggest that multiple F/T cycles had no effect on the ability of the ELISA assay to detect SARS-CoV-2 IgG antibodies. |*Blood Preservation[MESH] |*Cryopreservation[MESH] |Antibodies, Viral/*blood[MESH] |COVID-19 Serological Testing[MESH] |COVID-19/*diagnosis[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |Humans[MESH] |Immunoglobulin G/*blood[MESH] |SARS-CoV-2/immunology/*isolation & purification[MESH]Effect of multiple freeze-thaw cycles on the detection of anti-SARS-Co(epmc).pdf DeepDyve Pubget Overpricing