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10.1038/s41589-021-00842-2 http://scihub22266oqcxt.onion/10.1038/s41589-021-00842-2 34354262!10184463!34354262     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Chem+Biol 2021 ; 17 (9): 982-988 Nephropedia Template TP {{tp|p=34354262|t=2021. Accelerated RNA detection using tandem CRISPR nucleases |pdf=|usr=}}{{34354262}} gab.com Text Accelerated RNA detection using tandem CRISPR nucleases JO: Nat Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=34354262 #FOAvip Direct, amplification-free detection of RNA has the potential to transform molecular diagnostics by enabling simple on-site analysis of human or environmental samples. CRISPR-Cas nucleases offer programmable RNA-guided RNA recognition that triggers cleavage and release of a fluorescent reporter molecule, but long reaction times hamper their detection sensitivity and speed. Here, we show that unrelated CRISPR nucleases can be deployed in tandem to provide both direct RNA sensing and rapid signal generation, thus enabling robust detection of ~30 molecules per microl of RNA in 20 min. Combining RNA-guided Cas13 and Csm6 with a chemically stabilized activator creates a one-step assay that can detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA extracted from respiratory swab samples with quantitative reverse transcriptase PCR (qRT-PCR)-derived cycle threshold (C(t)) values up to 33, using a compact detector. This Fast Integrated Nuclease Detection In Tandem (FIND-IT) approach enables sensitive, direct RNA detection in a format that is amenable to point-of-care infection diagnosis as well as to a wide range of other diagnostic or research applications. Twit Text FOAVip Accelerated RNA detection using tandem CRISPR nucleases ????Nat Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=34354262 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34354262 #FOAvip English Wikipedia <ref>{{Cite pmid|34354262}}</ref> Accelerated RNA detection using tandem CRISPR nucleases #MMPMID34354262 Liu TY; Knott GJ; Smock DCJ; Desmarais JJ; Son S; Bhuiya A; Jakhanwal S; Prywes N; Agrawal S; Diaz de Leon Derby M; Switz NA; Armstrong M; Harris AR; Charles EJ; Thornton BW; Fozouni P; Shu J; Stephens SI; Kumar GR; Zhao C; Mok A; Iavarone AT; Escajeda AM; McIntosh R; Kim S; Dugan EJ; Pollard KS; Tan MX; Ott M; Fletcher DA; Lareau LF; Hsu PD; Savage DF; Doudna JA Nat Chem Biol 2021[Sep]; 17 (9): 982-988 PMID34354262show ga Direct, amplification-free detection of RNA has the potential to transform molecular diagnostics by enabling simple on-site analysis of human or environmental samples. CRISPR-Cas nucleases offer programmable RNA-guided RNA recognition that triggers cleavage and release of a fluorescent reporter molecule, but long reaction times hamper their detection sensitivity and speed. Here, we show that unrelated CRISPR nucleases can be deployed in tandem to provide both direct RNA sensing and rapid signal generation, thus enabling robust detection of ~30 molecules per microl of RNA in 20 min. Combining RNA-guided Cas13 and Csm6 with a chemically stabilized activator creates a one-step assay that can detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA extracted from respiratory swab samples with quantitative reverse transcriptase PCR (qRT-PCR)-derived cycle threshold (C(t)) values up to 33, using a compact detector. This Fast Integrated Nuclease Detection In Tandem (FIND-IT) approach enables sensitive, direct RNA detection in a format that is amenable to point-of-care infection diagnosis as well as to a wide range of other diagnostic or research applications. |COVID-19/*genetics[MESH] |CRISPR-Cas Systems/*genetics[MESH] |Humans[MESH] |RNA, Viral/*genetics[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH]Accelerated RNA detection using tandem CRISPR nucleases (epmc).pdf DeepDyve Pubget Overpricing