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10.1038/s41598-021-95563-w http://scihub22266oqcxt.onion/10.1038/s41598-021-95563-w 34354202!8342707!34354202     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 15869 Nephropedia Template TP {{tp|p=34354202|t=2021. Real-time SARS-CoV-2 diagnostic and variants tracking over multiple candidates using nanopore DNA sequencing |pdf=|usr=}}{{34354202}} gab.com Text Real-time SARS-CoV-2 diagnostic and variants tracking over multiple candidates using nanopore DNA sequencing JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34354202 #FOAvip Since December 2019, a novel coronavirus responsible for a severe acute respiratory syndrome (SARS-CoV-2) is accountable for a major pandemic situation. The emergence of the B.1.1.7 strain, as a highly transmissible variant has accelerated the world-wide interest in tracking SARS-CoV-2 variants' occurrence. Similarly, other extremely infectious variants, were described and further others are expected to be discovered due to the long period of time on which the pandemic situation is lasting. All described SARS-CoV-2 variants present several mutations within the gene encoding the Spike protein, involved in host receptor recognition and entry into the cell. Hence, instead of sequencing the whole viral genome for variants' tracking, herein we propose to focus on the SPIKE region to increase the number of candidate samples to screen at once; an essential aspect to accelerate diagnostics, but also variants' emergence/progression surveillance. This proof of concept study accomplishes both at once, population-scale diagnostics and variants' tracking. This strategy relies on (1) the use of the portable MinION DNA sequencer; (2) a DNA barcoding and a SPIKE gene-centered variant's tracking, increasing the number of candidates per assay; and (3) a real-time diagnostics and variant's tracking monitoring thanks to our software RETIVAD. This strategy represents an optimal solution for addressing the current needs on SARS-CoV-2 progression surveillance, notably due to its affordable implementation, allowing its implantation even in remote places over the world. Twit Text FOAVip Real-time SARS-CoV-2 diagnostic and variants tracking over multiple candidates using nanopore DNA sequencing ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34354202 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34354202 #FOAvip English Wikipedia <ref>{{Cite pmid|34354202}}</ref> Real-time SARS-CoV-2 diagnostic and variants tracking over multiple candidates using nanopore DNA sequencing #MMPMID34354202 Studer F; Petit JL; Engelen S; Mendoza-Parra MA Sci Rep 2021[Aug]; 11 (1): 15869 PMID34354202show ga Since December 2019, a novel coronavirus responsible for a severe acute respiratory syndrome (SARS-CoV-2) is accountable for a major pandemic situation. The emergence of the B.1.1.7 strain, as a highly transmissible variant has accelerated the world-wide interest in tracking SARS-CoV-2 variants' occurrence. Similarly, other extremely infectious variants, were described and further others are expected to be discovered due to the long period of time on which the pandemic situation is lasting. All described SARS-CoV-2 variants present several mutations within the gene encoding the Spike protein, involved in host receptor recognition and entry into the cell. Hence, instead of sequencing the whole viral genome for variants' tracking, herein we propose to focus on the SPIKE region to increase the number of candidate samples to screen at once; an essential aspect to accelerate diagnostics, but also variants' emergence/progression surveillance. This proof of concept study accomplishes both at once, population-scale diagnostics and variants' tracking. This strategy relies on (1) the use of the portable MinION DNA sequencer; (2) a DNA barcoding and a SPIKE gene-centered variant's tracking, increasing the number of candidates per assay; and (3) a real-time diagnostics and variant's tracking monitoring thanks to our software RETIVAD. This strategy represents an optimal solution for addressing the current needs on SARS-CoV-2 progression surveillance, notably due to its affordable implementation, allowing its implantation even in remote places over the world. |COVID-19 Nucleic Acid Testing/instrumentation/methods[MESH] |COVID-19/*diagnosis/virology[MESH] |Genome, Viral[MESH] |Humans[MESH] |Nanopores[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2/*genetics[MESH] |Sequence Analysis, DNA/instrumentation/*methods[MESH]Real-time SARS-CoV-2 diagnostic and variants tracking over multiple ca(epmc).pdf DeepDyve Pubget Overpricing