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10.1007/s42770-021-00556-6 http://scihub22266oqcxt.onion/10.1007/s42770-021-00556-6 34342836!8329412!34342836     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Braz+J+Microbiol 2021 ; 52 (4): 2069-2073 Nephropedia Template TP {{tp|p=34342836|t=2021. Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS-CoV-2 seroconversion |pdf=|usr=}}{{34342836}} gab.com Text Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS-CoV-2 seroconversion JO: Braz J Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=34342836 #FOAvip Serological assays are important tools to identify previous exposure to SARS-CoV-2, helping to track COVID-19 cases and determine the level of humoral response to SARS-CoV-2 infections and/or immunization to future vaccines. Here, the SARS-CoV-2 nucleocapsid protein was expressed in Escherichia coli and purified to homogeneity and high yield using a single chromatography step. The purified SARS-CoV-2 nucleocapsid protein was used to develop an indirect enzyme-linked immunosorbent assay for the identification of human SARS-CoV-2 seroconverts. The assay sensitivity and specificity were determined analyzing sera from 140 RT-qPCR-confirmed COVID-19 cases and 210 pre-pandemic controls. The assay operated with 90% sensitivity and 98% specificity; identical accuracies were obtained in head-to-head comparison with a commercial ELISA kit. Antigen-coated plates were stable for up to 3 months at 4 degrees C. The ELISA method described is ready for mass production and will be an additional tool to track COVID-19 cases. Twit Text FOAVip Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS-CoV-2 seroconversion ????Braz J Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=34342836 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34342836 #FOAvip English Wikipedia <ref>{{Cite pmid|34342836}}</ref> Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS-CoV-2 seroconversion #MMPMID34342836 Conzentino MS; Forchhammer K; Souza EM; Pedrosa FO; Nogueira MB; Raboni SM; Rego FGM; Zanette DL; Aoki MN; Nardin JM; Fornazari B; Morales HMP; Celedon PAF; Lima CVP; Mattar SB; Lin VH; Morello LG; Marchini FK; Reis RA; Huergo LF Braz J Microbiol 2021[Dec]; 52 (4): 2069-2073 PMID34342836show ga Serological assays are important tools to identify previous exposure to SARS-CoV-2, helping to track COVID-19 cases and determine the level of humoral response to SARS-CoV-2 infections and/or immunization to future vaccines. Here, the SARS-CoV-2 nucleocapsid protein was expressed in Escherichia coli and purified to homogeneity and high yield using a single chromatography step. The purified SARS-CoV-2 nucleocapsid protein was used to develop an indirect enzyme-linked immunosorbent assay for the identification of human SARS-CoV-2 seroconverts. The assay sensitivity and specificity were determined analyzing sera from 140 RT-qPCR-confirmed COVID-19 cases and 210 pre-pandemic controls. The assay operated with 90% sensitivity and 98% specificity; identical accuracies were obtained in head-to-head comparison with a commercial ELISA kit. Antigen-coated plates were stable for up to 3 months at 4 degrees C. The ELISA method described is ready for mass production and will be an additional tool to track COVID-19 cases. |*COVID-19/diagnosis/immunology[MESH] |*Enzyme-Linked Immunosorbent Assay[MESH] |*Seroconversion[MESH] |Antibodies, Viral/blood[MESH] |Coronavirus Nucleocapsid Proteins/*immunology[MESH] |Humans[MESH] |Immunity, Humoral[MESH] |Nucleocapsid Proteins/genetics[MESH] |Phosphoproteins/immunology[MESH]Antigen production and development of an indirect ELISA based on the n(epmc).pdf DeepDyve Pubget Overpricing