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10.1016/j.diamond.2021.108542 http://scihub22266oqcxt.onion/10.1016/j.diamond.2021.108542 34334952!8316675!34334952     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Diam+Relat+Mater 2021 ; 118 (ä): 108542 Nephropedia Template TP {{tp|p=34334952|t=2021. Boron doped diamond thin films for the electrochemical detection of SARS-CoV-2 S1 protein |pdf=|usr=}}{{34334952}} gab.com Text Boron doped diamond thin films for the electrochemical detection of SARS-CoV-2 S1 protein JO: Diam Relat Mater http://www.kidney.de/pget.php?&tw=1&pmid=34334952 #FOAvip Amidst a global pandemic, a precise and widely accessible rapid detection method is needed for accurate diagnosis and contact tracing. The lack of this technology was exposed through the outbreak of SARS-CoV-2 beginning in 2019. This study sets the foundation for the development of a boron doped diamond (BDD)-based impedimetric sensor. While specifically developed for use in the detection of SARS-CoV-2, this technology uses principles that could be adapted to detect other viruses in the future. Boron doped polycrystalline diamond electrodes were functionalized with a biotin-streptavidin linker complex and biotinylated anti-SARS-CoV-2 S1 antibodies. Electrodes were then incubated with the S1 subunit of the SARS-CoV-2 spike surface protein, and an electrical response was recorded using the changes to the electrode's charge transfer resistance (R(ct)), measured through electrochemical impedance spectroscopy (EIS). Detectable changes in the R(ct) were observed after 5-min incubation periods with S1 subunit concentrations as low as 1 fg/mL. Incubation with Influenza-B Hemagglutinin protein resulted in minimal change to the R(ct), indicating specificity of the BDD electrode for the S1 subunit of SARS-CoV-2. Detection of the S1 subunit in a complex (cell culture) medium was also demonstrated by modifying the EIS protocol to minimize the effects of sample matrix binding. BDD films of varying surface morphologies were investigated, and material characterization was used to give insight into the microstructure-performance relationship of the BDD sensing surface. Twit Text FOAVip Boron doped diamond thin films for the electrochemical detection of SARS-CoV-2 S1 protein ????Diam Relat Mater http://www.kidney.de/pget.php?&tw=1&pmid=34334952 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34334952 #FOAvip English Wikipedia <ref>{{Cite pmid|34334952}}</ref> Boron doped diamond thin films for the electrochemical detection of SARS-CoV-2 S1 protein #MMPMID34334952 Witt S; Rogien A; Werner D; Siegenthaler J; Lesiyon R; Kurien N; Rechenberg R; Baule N; Hardy A; Becker M Diam Relat Mater 2021[Oct]; 118 (ä): 108542 PMID34334952show ga Amidst a global pandemic, a precise and widely accessible rapid detection method is needed for accurate diagnosis and contact tracing. The lack of this technology was exposed through the outbreak of SARS-CoV-2 beginning in 2019. This study sets the foundation for the development of a boron doped diamond (BDD)-based impedimetric sensor. While specifically developed for use in the detection of SARS-CoV-2, this technology uses principles that could be adapted to detect other viruses in the future. Boron doped polycrystalline diamond electrodes were functionalized with a biotin-streptavidin linker complex and biotinylated anti-SARS-CoV-2 S1 antibodies. Electrodes were then incubated with the S1 subunit of the SARS-CoV-2 spike surface protein, and an electrical response was recorded using the changes to the electrode's charge transfer resistance (R(ct)), measured through electrochemical impedance spectroscopy (EIS). Detectable changes in the R(ct) were observed after 5-min incubation periods with S1 subunit concentrations as low as 1 fg/mL. Incubation with Influenza-B Hemagglutinin protein resulted in minimal change to the R(ct), indicating specificity of the BDD electrode for the S1 subunit of SARS-CoV-2. Detection of the S1 subunit in a complex (cell culture) medium was also demonstrated by modifying the EIS protocol to minimize the effects of sample matrix binding. BDD films of varying surface morphologies were investigated, and material characterization was used to give insight into the microstructure-performance relationship of the BDD sensing surface. äBoron doped diamond thin films for the electrochemical detection of SA(epmc).pdf DeepDyve Pubget Overpricing