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  • Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress #MMPMID34330917
  • Mendonca L; Howe A; Gilchrist JB; Sheng Y; Sun D; Knight ML; Zanetti-Domingues LC; Bateman B; Krebs AS; Chen L; Radecke J; Li VD; Ni T; Kounatidis I; Koronfel MA; Szynkiewicz M; Harkiolaki M; Martin-Fernandez ML; James W; Zhang P
  • Nat Commun 2021[Jul]; 12 (1): 4629 PMID34330917show ga
  • Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified viral components and inactivated viruses. However, structural and ultrastructural evidence on how the SARS-CoV-2 infection progresses in the native cellular context is scarce, and there is a lack of comprehensive knowledge on the SARS-CoV-2 replicative cycle. To correlate cytopathic events induced by SARS-CoV-2 with virus replication processes in frozen-hydrated cells, we established a unique multi-modal, multi-scale cryo-correlative platform to image SARS-CoV-2 infection in Vero cells. This platform combines serial cryoFIB/SEM volume imaging and soft X-ray cryo-tomography with cell lamellae-based cryo-electron tomography (cryoET) and subtomogram averaging. Here we report critical SARS-CoV-2 structural events - e.g. viral RNA transport portals, virus assembly intermediates, virus egress pathway, and native virus spike structures, in the context of whole-cell volumes revealing drastic cytppathic changes. This integrated approach allows a holistic view of SARS-CoV-2 infection, from the whole cell to individual molecules.
  • |Animals[MESH]
  • |COVID-19/epidemiology/*immunology/virology[MESH]
  • |Chlorocebus aethiops[MESH]
  • |Cryoelectron Microscopy[MESH]
  • |Electron Microscope Tomography[MESH]
  • |Humans[MESH]
  • |Pandemics/prevention & control[MESH]
  • |SARS-CoV-2/*immunology/physiology/ultrastructure[MESH]
  • |Vero Cells[MESH]
  • |Virus Assembly/*immunology/physiology[MESH]
  • |Virus Release/*immunology/physiology[MESH]
  • |Virus Replication/*immunology/physiology[MESH]

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  • suck abstract from ncbi

    4629 1.12 2021