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10.1038/s41467-021-24887-y http://scihub22266oqcxt.onion/10.1038/s41467-021-24887-y 34330917!8324836!34330917     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2021 ; 12 (1): 4629 Nephropedia Template TP {{tp|p=34330917|t=2021. Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress |pdf=|usr=}}{{34330917}} gab.com Text Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=34330917 #FOAvip Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified viral components and inactivated viruses. However, structural and ultrastructural evidence on how the SARS-CoV-2 infection progresses in the native cellular context is scarce, and there is a lack of comprehensive knowledge on the SARS-CoV-2 replicative cycle. To correlate cytopathic events induced by SARS-CoV-2 with virus replication processes in frozen-hydrated cells, we established a unique multi-modal, multi-scale cryo-correlative platform to image SARS-CoV-2 infection in Vero cells. This platform combines serial cryoFIB/SEM volume imaging and soft X-ray cryo-tomography with cell lamellae-based cryo-electron tomography (cryoET) and subtomogram averaging. Here we report critical SARS-CoV-2 structural events - e.g. viral RNA transport portals, virus assembly intermediates, virus egress pathway, and native virus spike structures, in the context of whole-cell volumes revealing drastic cytppathic changes. This integrated approach allows a holistic view of SARS-CoV-2 infection, from the whole cell to individual molecules. Twit Text FOAVip Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=34330917 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34330917 #FOAvip English Wikipedia <ref>{{Cite pmid|34330917}}</ref> Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress #MMPMID34330917 Mendonca L; Howe A; Gilchrist JB; Sheng Y; Sun D; Knight ML; Zanetti-Domingues LC; Bateman B; Krebs AS; Chen L; Radecke J; Li VD; Ni T; Kounatidis I; Koronfel MA; Szynkiewicz M; Harkiolaki M; Martin-Fernandez ML; James W; Zhang P Nat Commun 2021[Jul]; 12 (1): 4629 PMID34330917show ga Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified viral components and inactivated viruses. However, structural and ultrastructural evidence on how the SARS-CoV-2 infection progresses in the native cellular context is scarce, and there is a lack of comprehensive knowledge on the SARS-CoV-2 replicative cycle. To correlate cytopathic events induced by SARS-CoV-2 with virus replication processes in frozen-hydrated cells, we established a unique multi-modal, multi-scale cryo-correlative platform to image SARS-CoV-2 infection in Vero cells. This platform combines serial cryoFIB/SEM volume imaging and soft X-ray cryo-tomography with cell lamellae-based cryo-electron tomography (cryoET) and subtomogram averaging. Here we report critical SARS-CoV-2 structural events - e.g. viral RNA transport portals, virus assembly intermediates, virus egress pathway, and native virus spike structures, in the context of whole-cell volumes revealing drastic cytppathic changes. This integrated approach allows a holistic view of SARS-CoV-2 infection, from the whole cell to individual molecules. |Animals[MESH] |COVID-19/epidemiology/*immunology/virology[MESH] |Chlorocebus aethiops[MESH] |Cryoelectron Microscopy[MESH] |Electron Microscope Tomography[MESH] |Humans[MESH] |Pandemics/prevention & control[MESH] |SARS-CoV-2/*immunology/physiology/ultrastructure[MESH] |Vero Cells[MESH] |Virus Assembly/*immunology/physiology[MESH] |Virus Release/*immunology/physiology[MESH]Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and e(epmc).pdf DeepDyve Pubget Overpricing