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10.1002/anie.202107730 http://scihub22266oqcxt.onion/10.1002/anie.202107730 34328683!8426805!34328683     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Angew+Chem+Int+Ed+Engl 2021 ; 60 (39): 21211-21215 Nephropedia Template TP {{tp|p=34328683|t=2021. Discovery and Characterization of Spike N-Terminal Domain-Binding Aptamers for Rapid SARS-CoV-2 Detection |pdf=|usr=}}{{34328683}} gab.com Text Discovery and Characterization of Spike N-Terminal Domain-Binding Aptamers for Rapid SARS-CoV-2 Detection JO: Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=34328683 #FOAvip The coronavirus disease 2019 (COVID-19) pandemic has devastated families and disrupted healthcare, economies and societies across the globe. Molecular recognition agents that are specific for distinct viral proteins are critical components for rapid diagnostics and targeted therapeutics. In this work, we demonstrate the selection of novel DNA aptamers that bind to the SARS-CoV-2 spike glycoprotein with high specificity and affinity (<80 nM). Through binding assays and high resolution cryo-EM, we demonstrate that SNAP1 (SARS-CoV-2 spike protein N-terminal domain-binding aptamer 1) binds to the S N-terminal domain. We applied SNAP1 in lateral flow assays (LFAs) and ELISAs to detect UV-inactivated SARS-CoV-2 at concentrations as low as 5x10(5) copies mL(-1) . SNAP1 is therefore a promising molecular tool for SARS-CoV-2 diagnostics. Twit Text FOAVip Discovery and Characterization of Spike N-Terminal Domain-Binding Aptamers for Rapid SARS-CoV-2 Detection ????Angew Chem Int Ed Engl http://www.kidney.de/pget.php?&tw=1&pmid=34328683 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34328683 #FOAvip English Wikipedia <ref>{{Cite pmid|34328683}}</ref> Discovery and Characterization of Spike N-Terminal Domain-Binding Aptamers for Rapid SARS-CoV-2 Detection #MMPMID34328683 Kacherovsky N; Yang LF; Dang HV; Cheng EL; Cardle II; Walls AC; McCallum M; Sellers DL; DiMaio F; Salipante SJ; Corti D; Veesler D; Pun SH Angew Chem Int Ed Engl 2021[Sep]; 60 (39): 21211-21215 PMID34328683show ga The coronavirus disease 2019 (COVID-19) pandemic has devastated families and disrupted healthcare, economies and societies across the globe. Molecular recognition agents that are specific for distinct viral proteins are critical components for rapid diagnostics and targeted therapeutics. In this work, we demonstrate the selection of novel DNA aptamers that bind to the SARS-CoV-2 spike glycoprotein with high specificity and affinity (<80 nM). Through binding assays and high resolution cryo-EM, we demonstrate that SNAP1 (SARS-CoV-2 spike protein N-terminal domain-binding aptamer 1) binds to the S N-terminal domain. We applied SNAP1 in lateral flow assays (LFAs) and ELISAs to detect UV-inactivated SARS-CoV-2 at concentrations as low as 5x10(5) copies mL(-1) . SNAP1 is therefore a promising molecular tool for SARS-CoV-2 diagnostics. |Aptamers, Nucleotide/*chemistry[MESH] |COVID-19/*diagnosis/immunology[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |Humans[MESH] |Models, Molecular[MESH] |SARS-CoV-2/immunology/*isolation & purification[MESH]Discovery and Characterization of Spike N-Terminal Domain-Binding Apta(epmc).pdf DeepDyve Pubget Overpricing