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10.1002/jmv.27245 http://scihub22266oqcxt.onion/10.1002/jmv.27245 34324212!8426914!34324212     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 300.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 300.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2021 ; 93 (12): 6837-6840 Nephropedia Template TP {{tp|p=34324212|t=2021. Comparison of SARS-CoV-2 detection with the Cobas(R) 6800/8800 system on gargle samples using two sample processing methods with combined oropharyngeal/nasopharyngeal swab |pdf=|usr=}}{{34324212}} gab.com Text Comparison of SARS-CoV-2 detection with the Cobas(R) 6800/8800 system on gargle samples using two sample processing methods with combined oropharyngeal/nasopharyngeal swab JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=34324212 #FOAvip BACKGROUND: Gargle samples have been proposed as a noninvasive method for detection of SARS-CoV-2 RNA. The clinical performance of gargle specimens diluted in Cobas(R) PCR Media and in Cobas(R) Omni Lysis Reagent was compared to oropharyngeal/nasopharyngeal swab (ONPS) for the detection of SARS-CoV-2 RNA. STUDY DESIGN: Participants were recruited prospectively in two COVID-19 screening clinics. In addition to the ONPS, participants gargled with 5 ml of natural spring water split in the laboratory as follows: 1 ml was added to 4.3 ml of polymerase chain reaction (PCR) media and 400 mul was added to 200 mul of lysis buffer. Testing was performed with the Cobas(R) SARS-CoV-2 test on the Cobas(R) 6800 or 8800 platforms. RESULTS: Overall, 134/647 (20.7%) participants were considered infected because the ONPS or at least one gargle test was positive. ONPS had, respectively, a sensitivity of 96.3% (95% confidence interval [CI]: 91.3-98.5); both gargle processing methods were slightly less but equally sensitive (90.3% [95% CI: 83.9-94.3]). When ONPS and gargle specimens were both positive, the mean cycle threshold (C(t) ) was significantly higher for gargles, suggesting lower viral loads. CONCLUSION: Gargle specimens directly added in PCR Media provide a similar clinical sensitivity to chemical lysis, both having a slightly, not significantly, lower sensitivity to ONPS. Twit Text FOAVip Comparison of SARS-CoV-2 detection with the Cobas(R) 6800/8800 system on gargle samples using two sample processing methods with combined oropharyngeal/nasopharyngeal swab ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=34324212 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34324212 #FOAvip English Wikipedia <ref>{{Cite pmid|34324212}}</ref> Comparison of SARS-CoV-2 detection with the Cobas(R) 6800/8800 system on gargle samples using two sample processing methods with combined oropharyngeal/nasopharyngeal swab #MMPMID34324212 Benoit P; Labbe AC; Lalancette L; Gagnon S; Bonneau E; Lavallee C; Roger M; Fafard J; Dumaresq J; Beauchemin S; Coutlee F J Med Virol 2021[Dec]; 93 (12): 6837-6840 PMID34324212show ga BACKGROUND: Gargle samples have been proposed as a noninvasive method for detection of SARS-CoV-2 RNA. The clinical performance of gargle specimens diluted in Cobas(R) PCR Media and in Cobas(R) Omni Lysis Reagent was compared to oropharyngeal/nasopharyngeal swab (ONPS) for the detection of SARS-CoV-2 RNA. STUDY DESIGN: Participants were recruited prospectively in two COVID-19 screening clinics. In addition to the ONPS, participants gargled with 5 ml of natural spring water split in the laboratory as follows: 1 ml was added to 4.3 ml of polymerase chain reaction (PCR) media and 400 mul was added to 200 mul of lysis buffer. Testing was performed with the Cobas(R) SARS-CoV-2 test on the Cobas(R) 6800 or 8800 platforms. RESULTS: Overall, 134/647 (20.7%) participants were considered infected because the ONPS or at least one gargle test was positive. ONPS had, respectively, a sensitivity of 96.3% (95% confidence interval [CI]: 91.3-98.5); both gargle processing methods were slightly less but equally sensitive (90.3% [95% CI: 83.9-94.3]). When ONPS and gargle specimens were both positive, the mean cycle threshold (C(t) ) was significantly higher for gargles, suggesting lower viral loads. CONCLUSION: Gargle specimens directly added in PCR Media provide a similar clinical sensitivity to chemical lysis, both having a slightly, not significantly, lower sensitivity to ONPS. |COVID-19 Nucleic Acid Testing/*methods[MESH] |COVID-19/*diagnosis/*virology[MESH] |Diagnostic Tests, Routine/methods[MESH] |Humans[MESH] |Mass Screening/methods[MESH] |Nasopharynx/*virology[MESH] |Oropharynx/*virology[MESH] |Prospective Studies[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2/*genetics[MESH] |Saliva/virology[MESH] |Specimen Handling/methods[MESH]Comparison of SARS-CoV-2 detection with the Cobas(R) 6800/8800 system (epmc).pdf DeepDyve Pubget Overpricing