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10.15252/embj.2021108588 http://scihub22266oqcxt.onion/10.15252/embj.2021108588 34323299!8408615!34323299     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       EMBO+J 2021 ; 40 (17): e108588 Nephropedia Template TP {{tp|p=34323299|t=2021. A functional assay for serum detection of antibodies against SARS-CoV-2 nucleoprotein |pdf=|usr=}}{{34323299}} gab.com Text A functional assay for serum detection of antibodies against SARS-CoV-2 nucleoprotein JO: EMBO J http://www.kidney.de/pget.php?&tw=1&pmid=34323299 #FOAvip The humoral immune response to SARS-CoV-2 results in antibodies against spike (S) and nucleoprotein (N). However, whilst there are widely available neutralization assays for S antibodies, there is no assay for N-antibody activity. Here, we present a simple in vitro method called EDNA (electroporated-antibody-dependent neutralization assay) that provides a quantitative measure of N-antibody activity in unpurified serum from SARS-CoV-2 convalescents. We show that N antibodies neutralize SARS-CoV-2 intracellularly and cell-autonomously but require the cytosolic Fc receptor TRIM21. Using EDNA, we show that low N-antibody titres can be neutralizing, whilst some convalescents possess serum with high titres but weak activity. N-antibody and N-specific T-cell activity correlates within individuals, suggesting N antibodies may protect against SARS-CoV-2 by promoting antigen presentation. This work highlights the potential benefits of N-based vaccines and provides an in vitro assay to allow the antibodies they induce to be tested. Twit Text FOAVip A functional assay for serum detection of antibodies against SARS-CoV-2 nucleoprotein ????EMBO J http://www.kidney.de/pget.php?&tw=1&pmid=34323299 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34323299 #FOAvip English Wikipedia <ref>{{Cite pmid|34323299}}</ref> A functional assay for serum detection of antibodies against SARS-CoV-2 nucleoprotein #MMPMID34323299 Albecka A; Clift D; Vaysburd M; Rhinesmith T; Caddy SL; Favara DM; Baxendale HE; James LC EMBO J 2021[Sep]; 40 (17): e108588 PMID34323299show ga The humoral immune response to SARS-CoV-2 results in antibodies against spike (S) and nucleoprotein (N). However, whilst there are widely available neutralization assays for S antibodies, there is no assay for N-antibody activity. Here, we present a simple in vitro method called EDNA (electroporated-antibody-dependent neutralization assay) that provides a quantitative measure of N-antibody activity in unpurified serum from SARS-CoV-2 convalescents. We show that N antibodies neutralize SARS-CoV-2 intracellularly and cell-autonomously but require the cytosolic Fc receptor TRIM21. Using EDNA, we show that low N-antibody titres can be neutralizing, whilst some convalescents possess serum with high titres but weak activity. N-antibody and N-specific T-cell activity correlates within individuals, suggesting N antibodies may protect against SARS-CoV-2 by promoting antigen presentation. This work highlights the potential benefits of N-based vaccines and provides an in vitro assay to allow the antibodies they induce to be tested. |Antibodies, Neutralizing/blood/*isolation & purification[MESH] |Antibodies, Viral/blood/*isolation & purification[MESH] |COVID-19/*blood/virology[MESH] |Humans[MESH] |Nucleoproteins/blood/immunology[MESH]A functional assay for serum detection of antibodies against SARS-CoV-(epmc).pdf DeepDyve Pubget Overpricing