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10.1038/s41467-021-24874-3 http://scihub22266oqcxt.onion/10.1038/s41467-021-24874-3 34321486!8319308!34321486     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2021 ; 12 (1): 4586 Nephropedia Template TP {{tp|p=34321486|t=2021. A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays |pdf=|usr=}}{{34321486}} gab.com Text A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=34321486 #FOAvip Heterogeneous immunoassays such as ELISA have become indispensable in modern bioanalysis, yet translation into point-of-care assays is hindered by their dependence on external calibration and multiple washing and incubation steps. Here, we introduce RAPPID (Ratiometric Plug-and-Play Immunodiagnostics), a mix-and-measure homogeneous immunoassay platform that combines highly specific antibody-based detection with a ratiometric bioluminescent readout. The concept entails analyte-induced complementation of split NanoLuc luciferase fragments, photoconjugated to an antibody sandwich pair via protein G adapters. Introduction of a calibrator luciferase provides a robust ratiometric signal that allows direct in-sample calibration and quantitative measurements in complex media such as blood plasma. We developed RAPPID sensors that allow low-picomolar detection of several protein biomarkers, anti-drug antibodies, therapeutic antibodies, and both SARS-CoV-2 spike protein and anti-SARS-CoV-2 antibodies. With its easy-to-implement standardized workflow, RAPPID provides an attractive, fast, and low-cost alternative to traditional immunoassays, in an academic setting, in clinical laboratories, and for point-of-care applications. Twit Text FOAVip A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=34321486 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34321486 #FOAvip English Wikipedia <ref>{{Cite pmid|34321486}}</ref> A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays #MMPMID34321486 Ni Y; Rosier BJHM; van Aalen EA; Hanckmann ETL; Biewenga L; Pistikou AM; Timmermans B; Vu C; Roos S; Arts R; Li W; de Greef TFA; van Borren MMGJ; van Kuppeveld FJM; Bosch BJ; Merkx M Nat Commun 2021[Jul]; 12 (1): 4586 PMID34321486show ga Heterogeneous immunoassays such as ELISA have become indispensable in modern bioanalysis, yet translation into point-of-care assays is hindered by their dependence on external calibration and multiple washing and incubation steps. Here, we introduce RAPPID (Ratiometric Plug-and-Play Immunodiagnostics), a mix-and-measure homogeneous immunoassay platform that combines highly specific antibody-based detection with a ratiometric bioluminescent readout. The concept entails analyte-induced complementation of split NanoLuc luciferase fragments, photoconjugated to an antibody sandwich pair via protein G adapters. Introduction of a calibrator luciferase provides a robust ratiometric signal that allows direct in-sample calibration and quantitative measurements in complex media such as blood plasma. We developed RAPPID sensors that allow low-picomolar detection of several protein biomarkers, anti-drug antibodies, therapeutic antibodies, and both SARS-CoV-2 spike protein and anti-SARS-CoV-2 antibodies. With its easy-to-implement standardized workflow, RAPPID provides an attractive, fast, and low-cost alternative to traditional immunoassays, in an academic setting, in clinical laboratories, and for point-of-care applications. |Antibodies, Viral/*blood[MESH] |COVID-19 Serological Testing/instrumentation/*methods[MESH] |COVID-19/*diagnosis/immunology/virology[MESH] |Calibration[MESH] |GTP-Binding Proteins/chemistry[MESH] |Genes, Reporter[MESH] |Humans[MESH] |Immunoassay/*standards[MESH] |Immunoconjugates/chemistry[MESH] |Limit of Detection[MESH] |Luciferases/genetics/metabolism[MESH] |Luminescent Measurements/*standards[MESH] |Point-of-Care Testing[MESH] |SARS-CoV-2/genetics/*immunology[MESH]A plug-and-play platform of ratiometric bioluminescent sensors for hom(epmc).pdf DeepDyve Pubget Overpricing