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10.1126/science.abi9310 http://scihub22266oqcxt.onion/10.1126/science.abi9310 34315827!9836006!34315827     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Science 2021 ; 373 (6559): 1142-1146 Nephropedia Template TP {{tp|p=34315827|t=2021. Structural basis of mismatch recognition by a SARS-CoV-2 proofreading enzyme |pdf=|usr=}}{{34315827}} gab.com Text Structural basis of mismatch recognition by a SARS-CoV-2 proofreading enzyme JO: Science http://www.kidney.de/pget.php?&tw=1&pmid=34315827 #FOAvip Coronavirus 3'-to-5' exoribonuclease (ExoN), residing in the nonstructural protein (nsp) 10-nsp14 complex, boosts replication fidelity by proofreading RNA synthesis and is critical for the virus life cycle. ExoN also recognizes and excises nucleotide analog inhibitors incorporated into the nascent RNA, undermining the effectiveness of nucleotide analog-based antivirals. Here we present cryo-electron microscopy structures of both wild-type and mutant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nsp10-nsp14 in complex with an RNA substrate bearing a 3'-end mismatch at resolutions ranging from 2.5 to 3.9 angstroms. The structures reveal the molecular determinants of ExoN substrate specificity and offer insight into the molecular mechanisms of mismatch correction during coronavirus RNA synthesis. Our findings provide guidance for rational design of improved anticoronavirus therapies. Twit Text FOAVip Structural basis of mismatch recognition by a SARS-CoV-2 proofreading enzyme ????Science http://www.kidney.de/pget.php?&tw=1&pmid=34315827 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34315827 #FOAvip English Wikipedia <ref>{{Cite pmid|34315827}}</ref> Structural basis of mismatch recognition by a SARS-CoV-2 proofreading enzyme #MMPMID34315827 Liu C; Shi W; Becker ST; Schatz DG; Liu B; Yang Y Science 2021[Sep]; 373 (6559): 1142-1146 PMID34315827show ga Coronavirus 3'-to-5' exoribonuclease (ExoN), residing in the nonstructural protein (nsp) 10-nsp14 complex, boosts replication fidelity by proofreading RNA synthesis and is critical for the virus life cycle. ExoN also recognizes and excises nucleotide analog inhibitors incorporated into the nascent RNA, undermining the effectiveness of nucleotide analog-based antivirals. Here we present cryo-electron microscopy structures of both wild-type and mutant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nsp10-nsp14 in complex with an RNA substrate bearing a 3'-end mismatch at resolutions ranging from 2.5 to 3.9 angstroms. The structures reveal the molecular determinants of ExoN substrate specificity and offer insight into the molecular mechanisms of mismatch correction during coronavirus RNA synthesis. Our findings provide guidance for rational design of improved anticoronavirus therapies. |*DNA Mismatch Repair[MESH] |Antiviral Agents/chemistry/pharmacology[MESH] |Cryoelectron Microscopy[MESH] |Drug Design[MESH] |Exoribonucleases/*chemistry/genetics[MESH] |Humans[MESH] |Protein Domains[MESH] |RNA, Viral/biosynthesis/chemistry/genetics[MESH] |SARS-CoV-2/*enzymology/genetics[MESH] |Substrate Specificity[MESH] |Viral Nonstructural Proteins/*chemistry/genetics[MESH]Structural basis of mismatch recognition by a SARS-CoV-2 proofreading (epmc).pdf DeepDyve Pubget Overpricing