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10.1016/j.pdpdt.2021.102457 http://scihub22266oqcxt.onion/10.1016/j.pdpdt.2021.102457 34314863!8310417!34314863     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Photodiagnosis+Photodyn+Ther 2021 ; 35 (ä): 102457 Nephropedia Template TP {{tp|p=34314863|t=2021. Ultraviolet-A light reduces cellular cytokine release from human endotracheal cells infected with Coronavirus |pdf=|usr=}}{{34314863}} gab.com Text Ultraviolet-A light reduces cellular cytokine release from human endotracheal cells infected with Coronavirus JO: Photodiagnosis Photodyn Ther http://www.kidney.de/pget.php?&tw=1&pmid=34314863 #FOAvip BACKGROUND: An important clinical feature of coronavirus disease 2019 (COVID-19) is hypercytokinemia (cytokine storm). We previously showed that narrow band ultraviolet-A (NB-UVA) treatment salvages coronavirus (CoV)-229E-infected human tracheal cells, and that daily endotracheal NB-UVA therapy reduced severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) levels in human subjects, with improved clinical outcomes. Here, we examined NB-UVA effects on cytokine release during CoV-229E infection. METHODS: Primary human tracheal epithelial cells were transfected with CoV-229E, then exposed to 2 mW/cm(2) NB-UVA for 20 minutes every 24h, either 3 or 4 times. Secreted cytokine/chemokine levels were analyzed in supernatants collected from CoV-229E-infected/UVA-exposed cells 24h after the last UVA treatment, and from matched non-infected/UVA-exposed controls, CoV-229E-infected/non-exposed controls, and non-infected/non-exposed (naive) controls. Metabolic pathway/downstream prediction analyses were also performed. RESULTS: Pro-inflammatory cytokines interleukin (IL)-6 and tumor necrosis factor (TNF), and chemokines IL-8, monocyte chemoattractant protein-1 (MCP1), and interferon gamma-induced protein 10 (IP-10), were significantly increased in CoV-229E-infected cells, and significantly decreased following NB-UVA treatment. Interferon (IFN)-alpha2, IFN-gamma, and IL-10 were not upregulated in response to CoV-229E. Metabolic pathway predictions indicated hypercytokinemia as the top inflammatory response in CoV-229E-infected cells, whereas the top predicted pathway in CoV-229E-infected/UVA-exposed cells was the recovery stage of severe acute respiratory syndrome. CONCLUSIONS: Human tracheal epithelial cells infected with CoV-229E showed reduced cytokine secretions including IL-6, TNF, IL-8, and MCP-1, following NB-UVA exposure. This reduction of cytokine levels in vitro, coupled with previously identified reduced cell death in CoV-229E-infected/UVA-exposed cells, suggests that determining UVA effects on cytokine storm in human SARS-Co-V2 patients is warranted. Twit Text FOAVip Ultraviolet-A light reduces cellular cytokine release from human endotracheal cells infected with Coronavirus ????Photodiagnosis Photodyn Ther http://www.kidney.de/pget.php?&tw=1&pmid=34314863 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34314863 #FOAvip English Wikipedia <ref>{{Cite pmid|34314863}}</ref> Ultraviolet-A light reduces cellular cytokine release from human endotracheal cells infected with Coronavirus #MMPMID34314863 Leite G; Pimentel M; Mathur R; Barlow GM; Chan Y; Melmed GY; Rezaie A Photodiagnosis Photodyn Ther 2021[Sep]; 35 (ä): 102457 PMID34314863show ga BACKGROUND: An important clinical feature of coronavirus disease 2019 (COVID-19) is hypercytokinemia (cytokine storm). We previously showed that narrow band ultraviolet-A (NB-UVA) treatment salvages coronavirus (CoV)-229E-infected human tracheal cells, and that daily endotracheal NB-UVA therapy reduced severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) levels in human subjects, with improved clinical outcomes. Here, we examined NB-UVA effects on cytokine release during CoV-229E infection. METHODS: Primary human tracheal epithelial cells were transfected with CoV-229E, then exposed to 2 mW/cm(2) NB-UVA for 20 minutes every 24h, either 3 or 4 times. Secreted cytokine/chemokine levels were analyzed in supernatants collected from CoV-229E-infected/UVA-exposed cells 24h after the last UVA treatment, and from matched non-infected/UVA-exposed controls, CoV-229E-infected/non-exposed controls, and non-infected/non-exposed (naive) controls. Metabolic pathway/downstream prediction analyses were also performed. RESULTS: Pro-inflammatory cytokines interleukin (IL)-6 and tumor necrosis factor (TNF), and chemokines IL-8, monocyte chemoattractant protein-1 (MCP1), and interferon gamma-induced protein 10 (IP-10), were significantly increased in CoV-229E-infected cells, and significantly decreased following NB-UVA treatment. Interferon (IFN)-alpha2, IFN-gamma, and IL-10 were not upregulated in response to CoV-229E. Metabolic pathway predictions indicated hypercytokinemia as the top inflammatory response in CoV-229E-infected cells, whereas the top predicted pathway in CoV-229E-infected/UVA-exposed cells was the recovery stage of severe acute respiratory syndrome. CONCLUSIONS: Human tracheal epithelial cells infected with CoV-229E showed reduced cytokine secretions including IL-6, TNF, IL-8, and MCP-1, following NB-UVA exposure. This reduction of cytokine levels in vitro, coupled with previously identified reduced cell death in CoV-229E-infected/UVA-exposed cells, suggests that determining UVA effects on cytokine storm in human SARS-Co-V2 patients is warranted. |*COVID-19[MESH] |*Photochemotherapy/methods[MESH] |Cytokines[MESH] |Humans[MESH] |Photosensitizing Agents[MESH]Ultraviolet-A light reduces cellular cytokine release from human endot(epmc).pdf DeepDyve Pubget Overpricing