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10.1002/jmv.27240 http://scihub22266oqcxt.onion/10.1002/jmv.27240 34314050!8426954!34314050     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2021 ; 93 (12): 6833-6836 Nephropedia Template TP {{tp|p=34314050|t=2021. SARS-CoV-2 R 1 lineage variants that prevailed in Tokyo in March 2021 |pdf=|usr=}}{{34314050}} gab.com Text SARS-CoV-2 R 1 lineage variants that prevailed in Tokyo in March 2021 JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=34314050 #FOAvip The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants, such as B.1.1.7 and B.1.351, has become a crucial issue worldwide. Therefore, we began testing all patients with COVID-19 for the N501Y and E484K mutations by using polymerase chain reaction (PCR)-based methods. Nasopharyngeal swab samples from 108 patients who visited our hospital between February and April 2021 were analyzed. The samples were analyzed using reverse transcription-PCR with melting curve analysis to detect the N501Y and E484K mutations. A part of the samples was also subjected to whole-genome sequencing (WGS). Clinical parameters such as mortality and admission to the intensive care unit were analyzed to examine the association between increased disease severity and the E484K mutation. The ratio of cases showing the 501N + 484K mutation rapidly increased from 8% in February to 46% in March. WGS revealed that the viruses with 501N + 484K mutation are R.1 lineage variants. Evidence of increased disease severity related to the R.1 variants was not found. We found that the R.1 lineage variants rapidly prevailed in Tokyo in March 2021, which suggests the increased transmissibility of R.1 variants, while they showed no increased severity. Twit Text FOAVip SARS-CoV-2 R 1 lineage variants that prevailed in Tokyo in March 2021 ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=34314050 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34314050 #FOAvip English Wikipedia <ref>{{Cite pmid|34314050}}</ref> SARS-CoV-2 R 1 lineage variants that prevailed in Tokyo in March 2021 #MMPMID34314050 Nagano K; Tani-Sassa C; Iwasaki Y; Takatsuki Y; Yuasa S; Takahashi Y; Nakajima J; Sonobe K; Ichimura N; Nukui Y; Takeuchi H; Tanimoto K; Tanaka Y; Kimura A; Tohda S J Med Virol 2021[Dec]; 93 (12): 6833-6836 PMID34314050show ga The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants, such as B.1.1.7 and B.1.351, has become a crucial issue worldwide. Therefore, we began testing all patients with COVID-19 for the N501Y and E484K mutations by using polymerase chain reaction (PCR)-based methods. Nasopharyngeal swab samples from 108 patients who visited our hospital between February and April 2021 were analyzed. The samples were analyzed using reverse transcription-PCR with melting curve analysis to detect the N501Y and E484K mutations. A part of the samples was also subjected to whole-genome sequencing (WGS). Clinical parameters such as mortality and admission to the intensive care unit were analyzed to examine the association between increased disease severity and the E484K mutation. The ratio of cases showing the 501N + 484K mutation rapidly increased from 8% in February to 46% in March. WGS revealed that the viruses with 501N + 484K mutation are R.1 lineage variants. Evidence of increased disease severity related to the R.1 variants was not found. We found that the R.1 lineage variants rapidly prevailed in Tokyo in March 2021, which suggests the increased transmissibility of R.1 variants, while they showed no increased severity. |Aged[MESH] |COVID-19/*epidemiology/*virology[MESH] |Female[MESH] |Humans[MESH] |Male[MESH] |Mutation/genetics[MESH] |SARS-CoV-2/*genetics[MESH] |Spike Glycoprotein, Coronavirus/genetics[MESH] |Tokyo/epidemiology[MESH]SARS-CoV-2 R 1 lineage variants that prevailed in Tokyo in March 2021 (epmc).pdf DeepDyve Pubget Overpricing