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10.1038/s41598-021-94652-0

http://scihub22266oqcxt.onion/10.1038/s41598-021-94652-0
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34312441!8313664!34312441
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suck abstract from ncbi


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pmid34312441      Sci+Rep 2021 ; 11 (1): 15176
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  • An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection #MMPMID34312441
  • Deng H; Jayawardena A; Chan J; Tan SM; Alan T; Kwan P
  • Sci Rep 2021[Jul]; 11 (1): 15176 PMID34312441show ga
  • There is currently a high level of demand for rapid COVID-19 tests, that can detect the onset of the disease at point of care settings. We have developed an ultra-portable, self-contained, point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection, based on the principle of loop mediated isothermal amplification (LAMP). The LAMP assay is 100% sensitive and specific to detect a minimum of 300 RNA copies/reaction of SARS-CoV-2. All of the required sample transportation, lysing and amplification steps are performed in a standalone disposable cartridge, which is controlled by a battery operated, pocket size (6x9x4cm(3)) unit. The test is easy to operate and does not require skilled personnel. The total time from sample to answer is approximately 35 min; a colorimetric readout indicates positive or negative results. This portable diagnostic platform has significant potential for rapid and effective testing in community settings. This will accelerate clinical decision making, in terms of effective triage and timely therapeutic and infection control interventions.
  • |*Point-of-Care Testing/economics[MESH]
  • |COVID-19 Nucleic Acid Testing/economics/*instrumentation[MESH]
  • |COVID-19/*diagnosis/virology[MESH]
  • |Equipment Design[MESH]
  • |Humans[MESH]
  • |Molecular Diagnostic Techniques/economics/*instrumentation[MESH]
  • |Nucleic Acid Amplification Techniques/economics/*instrumentation[MESH]
  • |RNA, Viral/analysis/*genetics[MESH]
  • |SARS-CoV-2/*genetics/isolation & purification[MESH]
  • |Sensitivity and Specificity[MESH]


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