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10.1016/j.xpro.2021.100696 http://scihub22266oqcxt.onion/10.1016/j.xpro.2021.100696 34308375!8292114!34308375     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34308375&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       STAR+Protoc 2021 ; 2 (3): 100696 Nephropedia Template TP {{tp|p=34308375|t=2021. ACE2 chromogenic immunostaining protocol optimized for formalin-fixed paraffin-embedded human tissue sections |pdf=|usr=}}{{34308375}} gab.com Text ACE2 chromogenic immunostaining protocol optimized for formalin-fixed paraffin-embedded human tissue sections JO: STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=34308375 #FOAvip Angiotensin-converting enzyme 2 (ACE2) is a key cellular entry factor for severe acute respiratory syndrome coronavirus 2. Hence, identifying cell types that express ACE2 is important for understanding the pathophysiology of coronavirus disease 2019. We performed extensive testing of multiple primary antibodies across various human tissue types. Here, we describe an optimized protocol for immunostaining of ACE2 in formalin-fixed paraffin-embedded human pancreas, small intestine, and kidney tissue sections obtained from organ donors and autopsies. For complete details on the use and execution of this protocol, please refer to Kusmartseva et al. (2020). Twit Text FOAVip ACE2 chromogenic immunostaining protocol optimized for formalin-fixed paraffin-embedded human tissue sections ????STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=34308375 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34308375 #FOAvip English Wikipedia <ref>{{Cite pmid|34308375}}</ref> ACE2 chromogenic immunostaining protocol optimized for formalin-fixed paraffin-embedded human tissue sections #MMPMID34308375 Jorgensen M; Joseph P; Posgai AL; Vander Heide RS; Kusmartseva I; Atkinson MA STAR Protoc 2021[Sep]; 2 (3): 100696 PMID34308375show ga Angiotensin-converting enzyme 2 (ACE2) is a key cellular entry factor for severe acute respiratory syndrome coronavirus 2. Hence, identifying cell types that express ACE2 is important for understanding the pathophysiology of coronavirus disease 2019. We performed extensive testing of multiple primary antibodies across various human tissue types. Here, we describe an optimized protocol for immunostaining of ACE2 in formalin-fixed paraffin-embedded human pancreas, small intestine, and kidney tissue sections obtained from organ donors and autopsies. For complete details on the use and execution of this protocol, please refer to Kusmartseva et al. (2020). |Angiotensin-Converting Enzyme 2/*metabolism[MESH] |COVID-19/*diagnosis/metabolism/virology[MESH] |Formaldehyde/*chemistry[MESH] |Humans[MESH] |Immunohistochemistry/*methods[MESH] |Paraffin Embedding/*methods[MESH] |SARS-CoV-2/*isolation & purification[MESH]ACE2 chromogenic immunostaining protocol optimized for formalin-fixed (epmc).pdf DeepDyve Pubget Overpricing