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10.1016/j.mgene.2021.100950 http://scihub22266oqcxt.onion/10.1016/j.mgene.2021.100950 34307051!8286243!34307051     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Meta+Gene 2021 ; 30 (ä): 100950 Nephropedia Template TP {{tp|p=34307051|t=2021. Rapid, inexpensive methods for exploring SARS CoV-2 D614G mutation |pdf=|usr=}}{{34307051}} gab.com Text Rapid, inexpensive methods for exploring SARS CoV-2 D614G mutation JO: Meta Gene http://www.kidney.de/pget.php?&tw=1&pmid=34307051 #FOAvip A common mutation has occurred in the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS CoV-2), known as D614G (A23403G). There are discrepancies in the impact of this mutation on the virus's infectivity. Also, the whole genome sequencings are expensive and time-consuming. This study aims to develop three fast economical assays for prompt identifications of the D614G mutation including Taqman probe-based real-time reverse transcriptase polymerase chain reaction (rRT PCR), an amplification refractory mutation system (ARMS) RT and restriction fragment length polymorphism (RFLP), in nasopharyngeal swab samples. Both rRT and ARMS data showed G614 mutants indicated by the presence of HEX probe and 176 bp, respectively. Additionally, the results of the RFLP data and DNA sequencings confirmed the prevalence of the G614 mutants. These methods will be important, in epidemiological, reinfections and zoonotic aspects, through detecting the G614 mutant in retro-perspective samples to track its origins and future re-emergence of D614 wild type. Twit Text FOAVip Rapid, inexpensive methods for exploring SARS CoV-2 D614G mutation ????Meta Gene http://www.kidney.de/pget.php?&tw=1&pmid=34307051 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34307051 #FOAvip English Wikipedia <ref>{{Cite pmid|34307051}}</ref> Rapid, inexpensive methods for exploring SARS CoV-2 D614G mutation #MMPMID34307051 Al-Jaf SMA; Niranji SS; Mahmood ZH Meta Gene 2021[Dec]; 30 (ä): 100950 PMID34307051show ga A common mutation has occurred in the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS CoV-2), known as D614G (A23403G). There are discrepancies in the impact of this mutation on the virus's infectivity. Also, the whole genome sequencings are expensive and time-consuming. This study aims to develop three fast economical assays for prompt identifications of the D614G mutation including Taqman probe-based real-time reverse transcriptase polymerase chain reaction (rRT PCR), an amplification refractory mutation system (ARMS) RT and restriction fragment length polymorphism (RFLP), in nasopharyngeal swab samples. Both rRT and ARMS data showed G614 mutants indicated by the presence of HEX probe and 176 bp, respectively. Additionally, the results of the RFLP data and DNA sequencings confirmed the prevalence of the G614 mutants. These methods will be important, in epidemiological, reinfections and zoonotic aspects, through detecting the G614 mutant in retro-perspective samples to track its origins and future re-emergence of D614 wild type. äRapid, inexpensive methods for exploring SARS CoV-2 D614G mutation (epmc).pdf DeepDyve Pubget Overpricing