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10.1007/s12033-021-00373-0 http://scihub22266oqcxt.onion/10.1007/s12033-021-00373-0 34304364!8310559!34304364     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Biotechnol 2021 ; 63 (12): 1223-1234 Nephropedia Template TP {{tp|p=34304364|t=2021. Production of scFv, Fab, and IgG of CR3022 Antibodies Against SARS-CoV-2 Using Silkworm-Baculovirus Expression System |pdf=|usr=}}{{34304364}} gab.com Text Production of scFv, Fab, and IgG of CR3022 Antibodies Against SARS-CoV-2 Using Silkworm-Baculovirus Expression System JO: Mol Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=34304364 #FOAvip COVID-19, caused by SARS-CoV-2, is currently spreading around the world and causing many casualties. Antibodies against such emerging infectious diseases are one of the important tools for basic viral research and the development of diagnostic and therapeutic agents. CR3022 is a monoclonal antibody against the receptor binding domain (RBD) of the spike protein (S protein) of SARS-CoV found in SARS patients, but it was also shown to have strong affinity for that of SARS-CoV-2. In this study, we produced large amounts of three formats of CR3022 antibodies (scFv, Fab and IgG) with high purity using a silkworm-baculovirus expression vector system. Furthermore, SPR measurements showed that the affinity of those silkworm-produced IgG antibodies to S protein was almost the same as that produced in mammalian expression system. These results indicate that the silkworm-baculovirus expression system is an excellent expression system for emerging infectious diseases that require urgent demand for diagnostic agents and therapeutic agents. Twit Text FOAVip Production of scFv, Fab, and IgG of CR3022 Antibodies Against SARS-CoV-2 Using Silkworm-Baculovirus Expression System ????Mol Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=34304364 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34304364 #FOAvip English Wikipedia <ref>{{Cite pmid|34304364}}</ref> Production of scFv, Fab, and IgG of CR3022 Antibodies Against SARS-CoV-2 Using Silkworm-Baculovirus Expression System #MMPMID34304364 Ebihara T; Masuda A; Takahashi D; Hino M; Mon H; Kakino K; Fujii T; Fujita R; Ueda T; Lee JM; Kusakabe T Mol Biotechnol 2021[Dec]; 63 (12): 1223-1234 PMID34304364show ga COVID-19, caused by SARS-CoV-2, is currently spreading around the world and causing many casualties. Antibodies against such emerging infectious diseases are one of the important tools for basic viral research and the development of diagnostic and therapeutic agents. CR3022 is a monoclonal antibody against the receptor binding domain (RBD) of the spike protein (S protein) of SARS-CoV found in SARS patients, but it was also shown to have strong affinity for that of SARS-CoV-2. In this study, we produced large amounts of three formats of CR3022 antibodies (scFv, Fab and IgG) with high purity using a silkworm-baculovirus expression vector system. Furthermore, SPR measurements showed that the affinity of those silkworm-produced IgG antibodies to S protein was almost the same as that produced in mammalian expression system. These results indicate that the silkworm-baculovirus expression system is an excellent expression system for emerging infectious diseases that require urgent demand for diagnostic agents and therapeutic agents. |Animals[MESH] |Antibodies, Monoclonal/*biosynthesis/genetics[MESH] |Antibodies, Neutralizing/*biosynthesis/genetics[MESH] |Antibodies, Viral/*biosynthesis/genetics[MESH] |Antibody Affinity[MESH] |Baculoviridae/genetics/immunology[MESH] |Biotechnology[MESH] |Bombyx/genetics/immunology[MESH] |COVID-19/*immunology/*virology[MESH] |Cells, Cultured[MESH] |Gene Expression[MESH] |Hemolymph/immunology[MESH] |Humans[MESH] |Immunoglobulin Fab Fragments/biosynthesis/genetics[MESH] |Immunoglobulin Fragments/biosynthesis[MESH] |Immunoglobulin G/biosynthesis/genetics[MESH] |SARS-CoV-2/genetics/*immunology[MESH] |Single-Chain Antibodies/biosynthesis/genetics[MESH]Production of scFv, Fab, and IgG of CR3022 Antibodies Against SARS-CoV(epmc).pdf DeepDyve Pubget Overpricing