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10.1016/j.antiviral.2021.105142 http://scihub22266oqcxt.onion/10.1016/j.antiviral.2021.105142 34303749!8299157!34303749     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Antiviral+Res 2021 ; 193 (ä): 105142 Nephropedia Template TP {{tp|p=34303749|t=2021. Identification and evaluation of potential SARS-CoV-2 antiviral agents targeting mRNA cap guanine N7-Methyltransferase |pdf=|usr=}}{{34303749}} gab.com Text Identification and evaluation of potential SARS-CoV-2 antiviral agents targeting mRNA cap guanine N7-Methyltransferase JO: Antiviral Res http://www.kidney.de/pget.php?&tw=1&pmid=34303749 #FOAvip SARS-CoV-2, the cause of the currently ongoing COVID-19 pandemic, encodes its own mRNA capping machinery. Insights into this capping system may provide new ideas for therapeutic interventions and drug discovery. In this work, we employ a previously developed Py-FLINT screening approach to study the inhibitory effects of compounds against the cap guanine N7-methyltransferase enzyme, which is involved in SARS-CoV-2 mRNA capping. We screened five commercially available libraries (7039 compounds in total) to identify 83 inhibitors with IC(50) < 50 muM, which were further validated using RP HPLC and dot blot assays. Novel fluorescence anisotropy binding assays were developed to examine the targeted binding site. The inhibitor structures were analyzed for structure-activity relationships in order to define common structural patterns. Finally, the most potent inhibitors were tested for antiviral activity on SARS-CoV-2 in a cell based assay. Twit Text FOAVip Identification and evaluation of potential SARS-CoV-2 antiviral agents targeting mRNA cap guanine N7-Methyltransferase ????Antiviral Res http://www.kidney.de/pget.php?&tw=1&pmid=34303749 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34303749 #FOAvip English Wikipedia <ref>{{Cite pmid|34303749}}</ref> Identification and evaluation of potential SARS-CoV-2 antiviral agents targeting mRNA cap guanine N7-Methyltransferase #MMPMID34303749 Kasprzyk R; Spiewla TJ; Smietanski M; Golojuch S; Vangeel L; De Jonghe S; Jochmans D; Neyts J; Kowalska J; Jemielity J Antiviral Res 2021[Sep]; 193 (ä): 105142 PMID34303749show ga SARS-CoV-2, the cause of the currently ongoing COVID-19 pandemic, encodes its own mRNA capping machinery. Insights into this capping system may provide new ideas for therapeutic interventions and drug discovery. In this work, we employ a previously developed Py-FLINT screening approach to study the inhibitory effects of compounds against the cap guanine N7-methyltransferase enzyme, which is involved in SARS-CoV-2 mRNA capping. We screened five commercially available libraries (7039 compounds in total) to identify 83 inhibitors with IC(50) < 50 muM, which were further validated using RP HPLC and dot blot assays. Novel fluorescence anisotropy binding assays were developed to examine the targeted binding site. The inhibitor structures were analyzed for structure-activity relationships in order to define common structural patterns. Finally, the most potent inhibitors were tested for antiviral activity on SARS-CoV-2 in a cell based assay. |*COVID-19 Drug Treatment[MESH] |Antiviral Agents/chemistry/*pharmacology[MESH] |COVID-19/virology[MESH] |Cell Line[MESH] |Exoribonucleases/antagonists & inhibitors/metabolism[MESH] |High-Throughput Screening Assays[MESH] |Humans[MESH] |Inhibitory Concentration 50[MESH] |Methyltransferases/*antagonists & inhibitors/metabolism[MESH] |Nucleotidyltransferases/*antagonists & inhibitors/metabolism[MESH] |RNA Caps[MESH] |RNA, Viral/genetics/metabolism[MESH] |SARS-CoV-2/*drug effects/genetics/metabolism[MESH] |Viral Nonstructural Proteins/antagonists & inhibitors/metabolism[MESH]Identification and evaluation of potential SARS-CoV-2 antiviral agents(epmc).pdf DeepDyve Pubget Overpricing