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10.1007/s00705-021-05165-0 http://scihub22266oqcxt.onion/10.1007/s00705-021-05165-0 34302551!8302966!34302551     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Arch+Virol 2021 ; 166 (10): 2825-2828 Nephropedia Template TP {{tp|p=34302551|t=2021. Evaluation of extraction-free RT-qPCR methods for SARS-CoV-2 diagnostics |pdf=|usr=}}{{34302551}} gab.com Text Evaluation of extraction-free RT-qPCR methods for SARS-CoV-2 diagnostics JO: Arch Virol http://www.kidney.de/pget.php?&tw=1&pmid=34302551 #FOAvip Extraction-based real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) is currently the "gold standard" in SARS-CoV-2 diagnostics. However, some extraction-free RT-qPCR techniques have recently been developed. In this study, we compared the sensitivity of traditional extraction-based, heated extraction-free, and unheated extraction-free RT-qPCR methods for SARS-CoV-2 detection in nasopharyngeal swabs from symptomatic individuals. The unheated extraction-free method showed perfect agreement with the standard extraction-based RT-qPCR. By contrast, the heat-treated technique was associated with an 8.2% false negativity rate. Unheated extraction-free RT-qPCR for the molecular diagnosis of SARS-CoV-2 is a valuable alternative to the traditional extraction-based methods and may accelerate turnaround times by about two hours. Twit Text FOAVip Evaluation of extraction-free RT-qPCR methods for SARS-CoV-2 diagnostics ????Arch Virol http://www.kidney.de/pget.php?&tw=1&pmid=34302551 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34302551 #FOAvip English Wikipedia <ref>{{Cite pmid|34302551}}</ref> Evaluation of extraction-free RT-qPCR methods for SARS-CoV-2 diagnostics #MMPMID34302551 Domnich A; De Pace V; Pennati BM; Caligiuri P; Varesano S; Bruzzone B; Orsi A Arch Virol 2021[Oct]; 166 (10): 2825-2828 PMID34302551show ga Extraction-based real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) is currently the "gold standard" in SARS-CoV-2 diagnostics. However, some extraction-free RT-qPCR techniques have recently been developed. In this study, we compared the sensitivity of traditional extraction-based, heated extraction-free, and unheated extraction-free RT-qPCR methods for SARS-CoV-2 detection in nasopharyngeal swabs from symptomatic individuals. The unheated extraction-free method showed perfect agreement with the standard extraction-based RT-qPCR. By contrast, the heat-treated technique was associated with an 8.2% false negativity rate. Unheated extraction-free RT-qPCR for the molecular diagnosis of SARS-CoV-2 is a valuable alternative to the traditional extraction-based methods and may accelerate turnaround times by about two hours. |*COVID-19 Nucleic Acid Testing[MESH] |COVID-19/*diagnosis[MESH] |Humans[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2/genetics/*isolation & purification[MESH] |Sensitivity and Specificity[MESH]Evaluation of extraction-free RT-qPCR methods for SARS-CoV-2 diagnosti(epmc).pdf DeepDyve Pubget Overpricing