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10.1002/adma.202103221

http://scihub22266oqcxt.onion/10.1002/adma.202103221
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34302401!8440499!34302401
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suck abstract from ncbi


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pmid34302401      Adv+Mater 2021 ; 33 (37): e2103221
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  • High-Resolution Imaging of Human Viruses in Liquid Droplets #MMPMID34302401
  • Jonaid GM; Dearnaley WJ; Casasanta MA; Kaylor L; Berry S; Dukes MJ; Spilman MS; Gray JL; Kelly DF
  • Adv Mater 2021[Sep]; 33 (37): e2103221 PMID34302401show ga
  • Liquid-phase electron microscopy (LP-EM) is an exciting new area in the materials imaging field, providing unprecedented views of molecular processes. Time-resolved insights from LP-EM studies are a strong complement to the remarkable results achievable with other high-resolution techniques. Here, the opportunities to expand LP-EM technology beyond 2D temporal assessments and into the 3D regime are described. The results show new structures and dynamic insights of human viruses contained in minute volumes of liquid while acquired in a rapid timeframe. To develop this strategy, adeno-associated virus (AAV) is used as a model system. AAV is a well-known gene therapy vehicle with current applications involving drug delivery and vaccine development for COVID-19. Improving the understanding of the physical properties of biological entities in a liquid state, as maintained in the human body, has broad societal implications for human health and disease.
  • |*Dependovirus[MESH]
  • |*Particle Size[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Vaccines[MESH]
  • |Cryoelectron Microscopy/*methods[MESH]
  • |Drug Delivery Systems[MESH]
  • |Equipment Design[MESH]
  • |Genetic Therapy[MESH]
  • |HEK293 Cells/virology[MESH]
  • |Humans[MESH]
  • |Hydrogen-Ion Concentration[MESH]
  • |Immunoglobulin G/chemistry[MESH]
  • |Materials Testing[MESH]


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