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10.1002/adhm.202100410 http://scihub22266oqcxt.onion/10.1002/adhm.202100410 34297470!8427112!34297470     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Adv+Healthc+Mater 2021 ; 10 (18): e2100410 Nephropedia Template TP {{tp|p=34297470|t=2021. Enhancing the Stability of COVID-19 Serological Assay through Metal-Organic Framework Encapsulation |pdf=|usr=}}{{34297470}} gab.com Text Enhancing the Stability of COVID-19 Serological Assay through Metal-Organic Framework Encapsulation JO: Adv Healthc Mater http://www.kidney.de/pget.php?&tw=1&pmid=34297470 #FOAvip Enzyme-linked immunosorbent assay is widely utilized in serologic assays, including COVID-19, for the detection and quantification of antibodies against SARS-CoV-2. However, due to the limited stability of the diagnostic reagents (e.g., antigens serving as biorecognition elements) and biospecimens, temperature-controlled storage and handling conditions are critical. This limitation among others makes biodiagnostics in resource-limited settings, where refrigeration and electricity are inaccessible or unreliable, particularly challenging. In this work, metal-organic framework encapsulation is demonstrated as a simple and effective method to preserve the conformational epitopes of antigens immobilized on microtiter plate under non-refrigerated storage conditions. It is demonstrated that in situ growth of zeolitic imidazolate framework-90 (ZIF-90) renders excellent stability to surface-bound SARS-CoV-2 antigens, thereby maintaining the assay performance under elevated temperature (40 degrees C) for up to 4 weeks. As a complementary method, the preservation of plasma samples from COVID-19 patients using ZIF-90 encapsulation is also demonstrated. The energy-efficient approach demonstrated here will not only alleviate the financial burden associated with cold-chain transportation, but also improve the disease surveillance in resource-limited settings with more reliable clinical data. Twit Text FOAVip Enhancing the Stability of COVID-19 Serological Assay through Metal-Organic Framework Encapsulation ????Adv Healthc Mater http://www.kidney.de/pget.php?&tw=1&pmid=34297470 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34297470 #FOAvip English Wikipedia <ref>{{Cite pmid|34297470}}</ref> Enhancing the Stability of COVID-19 Serological Assay through Metal-Organic Framework Encapsulation #MMPMID34297470 Wang Y; Wang Z; Gupta P; Morrissey JJ; Naik RR; Singamaneni S Adv Healthc Mater 2021[Sep]; 10 (18): e2100410 PMID34297470show ga Enzyme-linked immunosorbent assay is widely utilized in serologic assays, including COVID-19, for the detection and quantification of antibodies against SARS-CoV-2. However, due to the limited stability of the diagnostic reagents (e.g., antigens serving as biorecognition elements) and biospecimens, temperature-controlled storage and handling conditions are critical. This limitation among others makes biodiagnostics in resource-limited settings, where refrigeration and electricity are inaccessible or unreliable, particularly challenging. In this work, metal-organic framework encapsulation is demonstrated as a simple and effective method to preserve the conformational epitopes of antigens immobilized on microtiter plate under non-refrigerated storage conditions. It is demonstrated that in situ growth of zeolitic imidazolate framework-90 (ZIF-90) renders excellent stability to surface-bound SARS-CoV-2 antigens, thereby maintaining the assay performance under elevated temperature (40 degrees C) for up to 4 weeks. As a complementary method, the preservation of plasma samples from COVID-19 patients using ZIF-90 encapsulation is also demonstrated. The energy-efficient approach demonstrated here will not only alleviate the financial burden associated with cold-chain transportation, but also improve the disease surveillance in resource-limited settings with more reliable clinical data. |*COVID-19[MESH] |*Metal-Organic Frameworks[MESH] |*Zeolites[MESH] |Antibodies[MESH] |Antibodies, Viral[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |Humans[MESH]Enhancing the Stability of COVID-19 Serological Assay through Metal-Or(epmc).pdf DeepDyve Pubget Overpricing