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10.1038/s41565-021-00939-8 http://scihub22266oqcxt.onion/10.1038/s41565-021-00939-8 34294909!8440422!34294909     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Nanotechnol 2021 ; 16 (9): 1039-1044 Nephropedia Template TP {{tp|p=34294909|t=2021. Liposome-mediated detection of SARS-CoV-2 RNA-positive extracellular vesicles in plasma |pdf=|usr=}}{{34294909}} gab.com Text Liposome-mediated detection of SARS-CoV-2 RNA-positive extracellular vesicles in plasma JO: Nat Nanotechnol http://www.kidney.de/pget.php?&tw=1&pmid=34294909 #FOAvip Plasma SARS-CoV-2 RNA may represent a viable diagnostic alternative to respiratory RNA levels, which rapidly decline after infection. Quantitative PCR with reverse transcription (RT-qPCR) reference assays exhibit poor performance with plasma, probably reflecting the dilution and degradation of viral RNA released into the circulation, but these issues could be addressed by analysing viral RNA packaged into extracellular vesicles. Here we describe an assay approach in which extracellular vesicles directly captured from plasma are fused with reagent-loaded liposomes to sensitively amplify and detect a SARS-CoV-2 gene target. This approach accurately identified patients with COVID-19, including challenging cases missed by RT-qPCR. SARS-CoV-2-positive extracellular vesicles were detected at day 1 post-infection, and plateaued from day 6 to the day 28 endpoint in a non-human primate model, while signal durations for 20-60 days were observed in young children. This nanotechnology approach uses a non-infectious sample and extends virus detection windows, offering a tool to support COVID-19 diagnosis in patients without SARS-CoV-2 RNA detectable in the respiratory tract. Twit Text FOAVip Liposome-mediated detection of SARS-CoV-2 RNA-positive extracellular vesicles in plasma ????Nat Nanotechnol http://www.kidney.de/pget.php?&tw=1&pmid=34294909 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34294909 #FOAvip English Wikipedia <ref>{{Cite pmid|34294909}}</ref> Liposome-mediated detection of SARS-CoV-2 RNA-positive extracellular vesicles in plasma #MMPMID34294909 Ning B; Huang Z; Youngquist BM; Scott JW; Niu A; Bojanowski CM; Zwezdaryk KJ; Saba NS; Fan J; Yin XM; Cao J; Lyon CJ; Li CZ; Roy CJ; Hu TY Nat Nanotechnol 2021[Sep]; 16 (9): 1039-1044 PMID34294909show ga Plasma SARS-CoV-2 RNA may represent a viable diagnostic alternative to respiratory RNA levels, which rapidly decline after infection. Quantitative PCR with reverse transcription (RT-qPCR) reference assays exhibit poor performance with plasma, probably reflecting the dilution and degradation of viral RNA released into the circulation, but these issues could be addressed by analysing viral RNA packaged into extracellular vesicles. Here we describe an assay approach in which extracellular vesicles directly captured from plasma are fused with reagent-loaded liposomes to sensitively amplify and detect a SARS-CoV-2 gene target. This approach accurately identified patients with COVID-19, including challenging cases missed by RT-qPCR. SARS-CoV-2-positive extracellular vesicles were detected at day 1 post-infection, and plateaued from day 6 to the day 28 endpoint in a non-human primate model, while signal durations for 20-60 days were observed in young children. This nanotechnology approach uses a non-infectious sample and extends virus detection windows, offering a tool to support COVID-19 diagnosis in patients without SARS-CoV-2 RNA detectable in the respiratory tract. |Animals[MESH] |Biosensing Techniques[MESH] |COVID-19 Nucleic Acid Testing[MESH] |COVID-19/blood/*diagnosis[MESH] |Chlorocebus aethiops[MESH] |Disease Models, Animal[MESH] |Extracellular Vesicles/*metabolism[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Kinetics[MESH] |Liposomes/metabolism/*therapeutic use[MESH] |RNA, Viral/*blood/genetics[MESH] |SARS-CoV-2/genetics/*isolation & purification[MESH]Liposome-mediated detection of SARS-CoV-2 RNA-positive extracellular v(epmc).pdf DeepDyve Pubget Overpricing