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10.2217/bmm-2020-0770 http://scihub22266oqcxt.onion/10.2217/bmm-2020-0770 34289718!8359910!34289718     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biomark+Med 2021 ; 15 (12): 987-997 Nephropedia Template TP {{tp|p=34289718|t=2021. Pre-analytical sample handling effects on blood cytokine levels: quality control of a COVID-19 biobank |pdf=|usr=}}{{34289718}} gab.com Text Pre-analytical sample handling effects on blood cytokine levels: quality control of a COVID-19 biobank JO: Biomark Med http://www.kidney.de/pget.php?&tw=1&pmid=34289718 #FOAvip Aim: We investigated the effect of pre-analytical sample handling variations on coronavirus disease 2019-relevant circulating cytokine levels IFN-gamma, IL-10, IL-12p70, IL-17A, IL-6 and TNF-alpha. Materials & methods: We collected blood in different collection tubes (ethylenediaminetetraacetic acid, sodium citrate, lithium heparin, serum), and subjected ethylenediaminetetraacetic acid plasma to among others increasing delays in centrifugation or -80 degrees C storage. Six subjects were included in each experimental condition. Cytokine levels were measured in these samples using the Simoa Cytokine 6-plex kit. Results: Different tube types resulted in different blood cytokine levels. IL-17A and IL-6 levels declined with 3 h centrifugation delay. IFN-gamma levels declined with 24 h postcentrifugation storage delay. IL-17A levels declined with 2-week storage delay. Conclusion: It is recommended to centrifuge tubes quickly following collection, for accurate cytokine measurement. Twit Text FOAVip Pre-analytical sample handling effects on blood cytokine levels: quality control of a COVID-19 biobank ????Biomark Med http://www.kidney.de/pget.php?&tw=1&pmid=34289718 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34289718 #FOAvip English Wikipedia <ref>{{Cite pmid|34289718}}</ref> Pre-analytical sample handling effects on blood cytokine levels: quality control of a COVID-19 biobank #MMPMID34289718 Verberk IM; Nossent EJ; Bontkes HJ; Teunissen CE Biomark Med 2021[Aug]; 15 (12): 987-997 PMID34289718show ga Aim: We investigated the effect of pre-analytical sample handling variations on coronavirus disease 2019-relevant circulating cytokine levels IFN-gamma, IL-10, IL-12p70, IL-17A, IL-6 and TNF-alpha. Materials & methods: We collected blood in different collection tubes (ethylenediaminetetraacetic acid, sodium citrate, lithium heparin, serum), and subjected ethylenediaminetetraacetic acid plasma to among others increasing delays in centrifugation or -80 degrees C storage. Six subjects were included in each experimental condition. Cytokine levels were measured in these samples using the Simoa Cytokine 6-plex kit. Results: Different tube types resulted in different blood cytokine levels. IL-17A and IL-6 levels declined with 3 h centrifugation delay. IFN-gamma levels declined with 24 h postcentrifugation storage delay. IL-17A levels declined with 2-week storage delay. Conclusion: It is recommended to centrifuge tubes quickly following collection, for accurate cytokine measurement. |*Quality Control[MESH] |Adult[MESH] |Biological Specimen Banks/*standards[MESH] |COVID-19/*blood[MESH] |Cytokines/*blood[MESH] |Female[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |SARS-CoV-2/*metabolism[MESH]Pre-analytical sample handling effects on blood cytokine levels: quali(epmc).pdf DeepDyve Pubget Overpricing