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10.1016/j.cell.2021.07.007 http://scihub22266oqcxt.onion/10.1016/j.cell.2021.07.007 34289344!8260498!34289344     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell 2021 ; 184 (17): 4392-4400.e4 Nephropedia Template TP {{tp|p=34289344|t=2021. A selective sweep in the Spike gene has driven SARS-CoV-2 human adaptation |pdf=|usr=}}{{34289344}} gab.com Text A selective sweep in the Spike gene has driven SARS-CoV-2 human adaptation JO: Cell http://www.kidney.de/pget.php?&tw=1&pmid=34289344 #FOAvip The coronavirus disease 2019 (COVID-19) pandemic underscores the need to better understand animal-to-human transmission of coronaviruses and adaptive evolution within new hosts. We scanned more than 182,000 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genomes for selective sweep signatures and found a distinct footprint of positive selection located around a non-synonymous change (A1114G; T372A) within the spike protein receptor-binding domain (RBD), predicted to remove glycosylation and increase binding to human ACE2 (hACE2), the cellular receptor. This change is present in all human SARS-CoV-2 sequences but not in closely related viruses from bats and pangolins. As predicted, T372A RBD bound hACE2 with higher affinity in experimental binding assays. We engineered the reversion mutant (A372T) and found that A372 (wild-type [WT]-SARS-CoV-2) enhanced replication in human lung cells relative to its putative ancestral variant (T372), an effect that was 20 times greater than the well-known D614G mutation. Our findings suggest that this mutation likely contributed to SARS-CoV-2 emergence from animal reservoirs or enabled sustained human-to-human transmission. Twit Text FOAVip A selective sweep in the Spike gene has driven SARS-CoV-2 human adaptation ????Cell http://www.kidney.de/pget.php?&tw=1&pmid=34289344 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34289344 #FOAvip English Wikipedia <ref>{{Cite pmid|34289344}}</ref> A selective sweep in the Spike gene has driven SARS-CoV-2 human adaptation #MMPMID34289344 Kang L; He G; Sharp AK; Wang X; Brown AM; Michalak P; Weger-Lucarelli J Cell 2021[Aug]; 184 (17): 4392-4400.e4 PMID34289344show ga The coronavirus disease 2019 (COVID-19) pandemic underscores the need to better understand animal-to-human transmission of coronaviruses and adaptive evolution within new hosts. We scanned more than 182,000 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genomes for selective sweep signatures and found a distinct footprint of positive selection located around a non-synonymous change (A1114G; T372A) within the spike protein receptor-binding domain (RBD), predicted to remove glycosylation and increase binding to human ACE2 (hACE2), the cellular receptor. This change is present in all human SARS-CoV-2 sequences but not in closely related viruses from bats and pangolins. As predicted, T372A RBD bound hACE2 with higher affinity in experimental binding assays. We engineered the reversion mutant (A372T) and found that A372 (wild-type [WT]-SARS-CoV-2) enhanced replication in human lung cells relative to its putative ancestral variant (T372), an effect that was 20 times greater than the well-known D614G mutation. Our findings suggest that this mutation likely contributed to SARS-CoV-2 emergence from animal reservoirs or enabled sustained human-to-human transmission. |Amino Acid Substitution[MESH] |Angiotensin-Converting Enzyme 2[MESH] |Animals[MESH] |COVID-19/*virology[MESH] |Cell Line[MESH] |Chiroptera/virology[MESH] |Chlorocebus aethiops[MESH] |Disease Reservoirs[MESH] |Evolution, Molecular[MESH] |Genome, Viral[MESH] |Humans[MESH] |Models, Molecular[MESH] |Mutation[MESH] |Phylogeny[MESH] |SARS-CoV-2/*genetics[MESH] |Spike Glycoprotein, Coronavirus/chemistry/*genetics/metabolism[MESH]A selective sweep in the Spike gene has driven SARS-CoV-2 human adapta(epmc).pdf DeepDyve Pubget Overpricing