Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 J+Am+Chem+Soc 2021 ; 143 (30): 11544-11553 Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Exponential Amplification Using Photoredox Autocatalysis #MMPMID34288684
Kim S; Martinez Dibildox A; Aguirre-Soto A; Sikes HD
J Am Chem Soc 2021[Aug]; 143 (30): 11544-11553 PMID34288684show ga
Exponential molecular amplification such as the polymerase chain reaction is a powerful tool that allows ultrasensitive biodetection. Here, we report a new exponential amplification strategy based on photoredox autocatalysis, where eosin Y, a photocatalyst, amplifies itself by activating a nonfluorescent eosin Y derivative (EYH(3-)) under green light. The deactivated photocatalyst is stable and rapidly activated under low-intensity light, making the eosin Y amplification suitable for resource-limited settings. Through steady-state kinetic studies and reaction modeling, we found that EYH(3-) is either oxidized to eosin Y via one-electron oxidation by triplet eosin Y and subsequent 1e(-)/H(+) transfer, or activated by singlet oxygen with the risk of degradation. By reducing the rate of the EYH(3-) degradation, we successfully improved EYH(3-)-to-eosin Y recovery, achieving efficient autocatalytic eosin Y amplification. Additionally, to demonstrate its flexibility in output signals, we coupled the eosin Y amplification with photoinduced chromogenic polymerization, enabling sensitive visual detection of analytes. Finally, we applied the exponential amplification methods in developing bioassays for detection of biomarkers including SARS-CoV-2 nucleocapsid protein, an antigen used in the diagnosis of COVID-19.
J+Am+Chem+Soc 2021 ; 143 (30): 11544-11553
