Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1021/jacs.1c03003 http://scihub22266oqcxt.onion/10.1021/jacs.1c03003 34283611!8315264!34283611     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Am+Chem+Soc 2021 ; 143 (30): 11404-11422 Nephropedia Template TP {{tp|p=34283611|t=2021. To Knot or Not to Knot: Multiple Conformations of the SARS-CoV-2 Frameshifting RNA Element |pdf=|usr=}}{{34283611}} gab.com Text To Knot or Not to Knot: Multiple Conformations of the SARS-CoV-2 Frameshifting RNA Element JO: J Am Chem Soc http://www.kidney.de/pget.php?&tw=1&pmid=34283611 #FOAvip The SARS-CoV-2 frameshifting RNA element (FSE) is an excellent target for therapeutic intervention against Covid-19. This small gene element employs a shifting mechanism to pause and backtrack the ribosome during translation between Open Reading Frames 1a and 1b, which code for viral polyproteins. Any interference with this process has a profound effect on viral replication and propagation. Pinpointing the structures adapted by the FSE and associated structural transformations involved in frameshifting has been a challenge. Using our graph-theory-based modeling tools for representing RNA secondary structures, "RAG" (RNA-As-Graphs), and chemical structure probing experiments, we show that the 3-stem H-type pseudoknot (3_6 dual graph), long assumed to be the dominant structure, has a viable alternative, an HL-type 3-stem pseudoknot (3_3) for longer constructs. In addition, an unknotted 3-way junction RNA (3_5) emerges as a minor conformation. These three conformations share Stems 1 and 3, while the different Stem 2 may be involved in a conformational switch and possibly associations with the ribosome during translation. For full-length genomes, a stem-loop motif (2_2) may compete with these forms. These structural and mechanistic insights advance our understanding of the SARS-CoV-2 frameshifting process and concomitant virus life cycle, and point to three avenues of therapeutic intervention. Twit Text FOAVip To Knot or Not to Knot: Multiple Conformations of the SARS-CoV-2 Frameshifting RNA Element ????J Am Chem Soc http://www.kidney.de/pget.php?&tw=1&pmid=34283611 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34283611 #FOAvip English Wikipedia <ref>{{Cite pmid|34283611}}</ref> To Knot or Not to Knot: Multiple Conformations of the SARS-CoV-2 Frameshifting RNA Element #MMPMID34283611 Schlick T; Zhu Q; Dey A; Jain S; Yan S; Laederach A J Am Chem Soc 2021[Aug]; 143 (30): 11404-11422 PMID34283611show ga The SARS-CoV-2 frameshifting RNA element (FSE) is an excellent target for therapeutic intervention against Covid-19. This small gene element employs a shifting mechanism to pause and backtrack the ribosome during translation between Open Reading Frames 1a and 1b, which code for viral polyproteins. Any interference with this process has a profound effect on viral replication and propagation. Pinpointing the structures adapted by the FSE and associated structural transformations involved in frameshifting has been a challenge. Using our graph-theory-based modeling tools for representing RNA secondary structures, "RAG" (RNA-As-Graphs), and chemical structure probing experiments, we show that the 3-stem H-type pseudoknot (3_6 dual graph), long assumed to be the dominant structure, has a viable alternative, an HL-type 3-stem pseudoknot (3_3) for longer constructs. In addition, an unknotted 3-way junction RNA (3_5) emerges as a minor conformation. These three conformations share Stems 1 and 3, while the different Stem 2 may be involved in a conformational switch and possibly associations with the ribosome during translation. For full-length genomes, a stem-loop motif (2_2) may compete with these forms. These structural and mechanistic insights advance our understanding of the SARS-CoV-2 frameshifting process and concomitant virus life cycle, and point to three avenues of therapeutic intervention. |Base Sequence[MESH] |Inverted Repeat Sequences[MESH] |Models, Molecular[MESH] |Nucleic Acid Conformation[MESH] |RNA, Viral/*chemistry/genetics[MESH]To Knot or Not to Knot: Multiple Conformations of the SARS-CoV-2 Frame(epmc).pdf DeepDyve Pubget Overpricing