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10.1016/j.diagmicrobio.2021.115469 http://scihub22266oqcxt.onion/10.1016/j.diagmicrobio.2021.115469 34280773!8230941!34280773     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Diagn+Microbiol+Infect+Dis 2021 ; 101 (2): 115469 Nephropedia Template TP {{tp|p=34280773|t=2021. Practical strategies for SARS-CoV-2 RT-PCR testing in resource-constrained settings |pdf=|usr=}}{{34280773}} gab.com Text Practical strategies for SARS-CoV-2 RT-PCR testing in resource-constrained settings JO: Diagn Microbiol Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=34280773 #FOAvip Alternatives to nasopharyngeal sampling are needed to increase capacity for SARS-CoV-2 testing. Among 275 participants, we piloted the collection of nasal mid-turbinate swabs amenable to self-testing, including polyester flocked swabs as well as 3D-printed plastic lattice swabs, placed into viral transport media or an RNA stabilization agent. Flocked nasal swabs identified 104/121 individuals who were PCR-positive for SARS-CoV-2 by nasopharyngeal sampling (sensitivity 87%, 95% CI 79-92%), missing those with low viral load (<10(6) viral copies/mL). 3D-printed nasal swabs showed similar sensitivity. When nasal swabs were placed directly into RNA preservative, the mean 1.4 log decrease in viral copies/uL compared to nasopharyngeal samples was reduced to <1 log, even when samples were left at room temperature for up to 7 days. We also evaluated pooling strategies that involved pooling specimens in the lab versus pooling swabs at the point of collection, finding both successfully detected samples with >10(5) viral copies/mL. Twit Text FOAVip Practical strategies for SARS-CoV-2 RT-PCR testing in resource-constrained settings ????Diagn Microbiol Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=34280773 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34280773 #FOAvip English Wikipedia <ref>{{Cite pmid|34280773}}</ref> Practical strategies for SARS-CoV-2 RT-PCR testing in resource-constrained settings #MMPMID34280773 Muller MS; Chhetri SB; Basham C; Rapp T; Lin FC; Lin K; Westreich D; Cerami C; Juliano JJ; Lin JT Diagn Microbiol Infect Dis 2021[Oct]; 101 (2): 115469 PMID34280773show ga Alternatives to nasopharyngeal sampling are needed to increase capacity for SARS-CoV-2 testing. Among 275 participants, we piloted the collection of nasal mid-turbinate swabs amenable to self-testing, including polyester flocked swabs as well as 3D-printed plastic lattice swabs, placed into viral transport media or an RNA stabilization agent. Flocked nasal swabs identified 104/121 individuals who were PCR-positive for SARS-CoV-2 by nasopharyngeal sampling (sensitivity 87%, 95% CI 79-92%), missing those with low viral load (<10(6) viral copies/mL). 3D-printed nasal swabs showed similar sensitivity. When nasal swabs were placed directly into RNA preservative, the mean 1.4 log decrease in viral copies/uL compared to nasopharyngeal samples was reduced to <1 log, even when samples were left at room temperature for up to 7 days. We also evaluated pooling strategies that involved pooling specimens in the lab versus pooling swabs at the point of collection, finding both successfully detected samples with >10(5) viral copies/mL. |COVID-19 Nucleic Acid Testing/*methods[MESH] |COVID-19/*diagnosis[MESH] |Health Resources/supply & distribution[MESH] |Humans[MESH] |Limit of Detection[MESH] |Nasopharynx/virology[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2/genetics/*isolation & purification[MESH] |Self-Testing[MESH] |Specimen Handling/instrumentation/methods[MESH] |Turbinates/virology[MESH]Practical strategies for SARS-CoV-2 RT-PCR testing in resource-constra(epmc).pdf DeepDyve Pubget Overpricing