Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1002/advs.202100323 http://scihub22266oqcxt.onion/10.1002/advs.202100323 34278739!8456206!34278739     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Adv+Sci+(Weinh) 2021 ; 8 (18): e2100323 Nephropedia Template TP {{tp|p=34278739|t=2021. Cell Analysis from Dried Blood Spots: New Opportunities in Immunology, Hematology, and Infectious Diseases |pdf=|usr=}}{{34278739}} gab.com Text Cell Analysis from Dried Blood Spots: New Opportunities in Immunology, Hematology, and Infectious Diseases JO: Adv Sci (Weinh) http://www.kidney.de/pget.php?&tw=1&pmid=34278739 #FOAvip Blood cell analysis is a major pillar of biomedical research and healthcare. These analyses are performed in central laboratories. Rapid shipment from collection site to the central laboratories is currently needed because cells and biomarkers degrade rapidly. The dried blood spot from a fingerstick allows the preservation of cellular molecules for months but entire cells are never recovered. Here leucocyte elution is optimized from dried blood spots. Flow cytometry and mRNA expression profiling are used to analyze the recovered cells. 50-70% of the leucocytes that are dried on a polyester solid support via elution after shaking the support with buffer are recovered. While red blood cells lyse upon drying, it is found that the majority of leucocytes are preserved. Leucocytes have an altered structure that is improved by adding fixative in the elution buffer. Leucocytes are permeabilized, allowing an easy staining of all cellular compartments. Common immunophenotyping and mRNAs are preserved. The ability of a new biomarker (CD169) to discriminate between patients with and without Severe Acute Respiratory Syndrome induced by Coronavirus 2 (SARS-CoV-2) infections is also preserved. Leucocytes from blood can be dried, shipped, and/or stored for at least 1 month, then recovered for a wide variety of analyses, potentially facilitating biomedical applications worldwide. Twit Text FOAVip Cell Analysis from Dried Blood Spots: New Opportunities in Immunology, Hematology, and Infectious Diseases ????Adv Sci (Weinh) http://www.kidney.de/pget.php?&tw=1&pmid=34278739 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34278739 #FOAvip English Wikipedia <ref>{{Cite pmid|34278739}}</ref> Cell Analysis from Dried Blood Spots: New Opportunities in Immunology, Hematology, and Infectious Diseases #MMPMID34278739 Ait Belkacem I; Mossadegh-Keller N; Bourgoin P; Arnoux I; Loosveld M; Morange PE; Markarian T; Michelet P; Busnel JM; Roulland S; Galland F; Malergue F Adv Sci (Weinh) 2021[Sep]; 8 (18): e2100323 PMID34278739show ga Blood cell analysis is a major pillar of biomedical research and healthcare. These analyses are performed in central laboratories. Rapid shipment from collection site to the central laboratories is currently needed because cells and biomarkers degrade rapidly. The dried blood spot from a fingerstick allows the preservation of cellular molecules for months but entire cells are never recovered. Here leucocyte elution is optimized from dried blood spots. Flow cytometry and mRNA expression profiling are used to analyze the recovered cells. 50-70% of the leucocytes that are dried on a polyester solid support via elution after shaking the support with buffer are recovered. While red blood cells lyse upon drying, it is found that the majority of leucocytes are preserved. Leucocytes have an altered structure that is improved by adding fixative in the elution buffer. Leucocytes are permeabilized, allowing an easy staining of all cellular compartments. Common immunophenotyping and mRNAs are preserved. The ability of a new biomarker (CD169) to discriminate between patients with and without Severe Acute Respiratory Syndrome induced by Coronavirus 2 (SARS-CoV-2) infections is also preserved. Leucocytes from blood can be dried, shipped, and/or stored for at least 1 month, then recovered for a wide variety of analyses, potentially facilitating biomedical applications worldwide. |Antibodies, Viral/blood[MESH] |Biomarkers/blood[MESH] |Blood Specimen Collection/methods[MESH] |COVID-19/diagnosis[MESH] |Cell Separation/methods[MESH] |Communicable Diseases/*diagnosis/virology[MESH] |Diagnostic Tests, Routine/*methods[MESH] |Dried Blood Spot Testing/*methods[MESH] |Erythrocytes/virology[MESH] |Flow Cytometry/methods[MESH] |Hematology/*methods[MESH] |Humans[MESH] |Immunophenotyping/*methods[MESH] |Leukocytes/virology[MESH] |RNA, Messenger/blood[MESH]Cell Analysis from Dried Blood Spots: New Opportunities in Immunology,(epmc).pdf DeepDyve Pubget Overpricing