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10.1186/s12985-021-01620-1 http://scihub22266oqcxt.onion/10.1186/s12985-021-01620-1 34275492!8286443!34275492     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34275492&cmd=llinks): Failed to open stream: HTTP request failed! 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SARS-CoV-2: preliminary study of infected human nasopharyngeal tissue by high resolution microscopy |pdf=|usr=}}{{34275492}} gab.com Text SARS-CoV-2: preliminary study of infected human nasopharyngeal tissue by high resolution microscopy JO: Virol J http://www.kidney.de/pget.php?&tw=1&pmid=34275492 #FOAvip BACKGROUND: The novel coronavirus SARS-CoV-2 is the etiological agent of COVID-19. This virus has become one of the most dangerous in recent times with a very high rate of transmission. At present, several publications show the typical crown-shape of the novel coronavirus grown in cell cultures. However, an integral ultramicroscopy study done directly from clinical specimens has not been published. METHODS: Nasopharyngeal swabs were collected from 12 Cuban individuals, six asymptomatic and RT-PCR negative (negative control) and six others from a COVID-19 symptomatic and RT-PCR positive for SARS CoV-2. Samples were treated with an aldehyde solution and processed by scanning electron microscopy (SEM), confocal microscopy (CM) and, atomic force microscopy. Improvement and segmentation of coronavirus images were performed by a novel mathematical image enhancement algorithm. RESULTS: The images of the negative control sample showed the characteristic healthy microvilli morphology at the apical region of the nasal epithelial cells. As expected, they do not display virus-like structures. The images of the positive sample showed characteristic coronavirus-like particles and evident destruction of microvilli. In some regions, virions budding through the cell membrane were observed. Microvilli destruction could explain the anosmia reported by some patients. Virus-particles emerging from the cell-surface with a variable size ranging from 80 to 400 nm were observed by SEM. Viral antigen was identified in the apical cells zone by CM. CONCLUSIONS: The integral microscopy study showed that SARS-CoV-2 has a similar image to SARS-CoV. The application of several high-resolution microscopy techniques to nasopharyngeal samples awaits future use. Twit Text FOAVip SARS-CoV-2: preliminary study of infected human nasopharyngeal tissue by high resolution microscopy ????Virol J http://www.kidney.de/pget.php?&tw=1&pmid=34275492 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34275492 #FOAvip English Wikipedia <ref>{{Cite pmid|34275492}}</ref> SARS-CoV-2: preliminary study of infected human nasopharyngeal tissue by high resolution microscopy #MMPMID34275492 Mondeja B; Valdes O; Resik S; Vizcaino A; Acosta E; Montalvan A; Paez A; Mune M; Rodriguez R; Valdes J; Gonzalez G; Sanchez D; Falcon V; Gonzalez Y; Kouri V; Diaz A; Guzman M Virol J 2021[Jul]; 18 (1): 149 PMID34275492show ga BACKGROUND: The novel coronavirus SARS-CoV-2 is the etiological agent of COVID-19. This virus has become one of the most dangerous in recent times with a very high rate of transmission. At present, several publications show the typical crown-shape of the novel coronavirus grown in cell cultures. However, an integral ultramicroscopy study done directly from clinical specimens has not been published. METHODS: Nasopharyngeal swabs were collected from 12 Cuban individuals, six asymptomatic and RT-PCR negative (negative control) and six others from a COVID-19 symptomatic and RT-PCR positive for SARS CoV-2. Samples were treated with an aldehyde solution and processed by scanning electron microscopy (SEM), confocal microscopy (CM) and, atomic force microscopy. Improvement and segmentation of coronavirus images were performed by a novel mathematical image enhancement algorithm. RESULTS: The images of the negative control sample showed the characteristic healthy microvilli morphology at the apical region of the nasal epithelial cells. As expected, they do not display virus-like structures. The images of the positive sample showed characteristic coronavirus-like particles and evident destruction of microvilli. In some regions, virions budding through the cell membrane were observed. Microvilli destruction could explain the anosmia reported by some patients. Virus-particles emerging from the cell-surface with a variable size ranging from 80 to 400 nm were observed by SEM. Viral antigen was identified in the apical cells zone by CM. CONCLUSIONS: The integral microscopy study showed that SARS-CoV-2 has a similar image to SARS-CoV. The application of several high-resolution microscopy techniques to nasopharyngeal samples awaits future use. |Antigens, Viral/metabolism[MESH] |COVID-19/diagnosis/*pathology/virology[MESH] |Epithelial Cells/ultrastructure/virology[MESH] |Humans[MESH] |Image Enhancement[MESH] |Microscopy[MESH] |Microvilli/ultrastructure[MESH] |Nasal Mucosa/ultrastructure/virology[MESH] |Nasopharynx/*ultrastructure/virology[MESH] |SARS-CoV-2/isolation & purification/*ultrastructure[MESH]SARS-CoV-2: preliminary study of infected human nasopharyngeal tissue (epmc).pdf DeepDyve Pubget Overpricing