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10.1016/j.diagmicrobio.2021.115458 http://scihub22266oqcxt.onion/10.1016/j.diagmicrobio.2021.115458 34274751!8222080!34274751     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Diagn+Microbiol+Infect+Dis 2021 ; 101 (2): 115458 Nephropedia Template TP {{tp|p=34274751|t=2021. Extractionless nucleic acid detection: a high capacity solution to COVID-19 testing |pdf=|usr=}}{{34274751}} gab.com Text Extractionless nucleic acid detection: a high capacity solution to COVID-19 testing JO: Diagn Microbiol Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=34274751 #FOAvip We describe an extractionless real-time reverse transcriptase-PCR (rRT-PCR) protocol for SARS-CoV-2 nucleic acid detection using heat as an accurate cost-effective high-capacity solution to COVID-19 testing. We present the effect of temperature, transport media, rRT-PCR mastermixes and gene assays on SARS-CoV-2 gene amplification and limits of detection. Utilizing our heated methodology, our limits of detection were 12.5 and 1 genome copy/reaction for singleplex E- and N1-gene assays, respectively, and 1 genome copy/reaction by utilizing an E/N1 or Orf1ab/N1 multiplex assay combination. Using this approach, we detected up to 98% of COVID-19 positive patient samples analyzed in our various cohorts including a significant percentage of weak positives. Importantly, this extractionless approach will allow for >2-fold increase in testing capacity with existing instruments, circumvent the additional need for expensive extraction devices, provide the sensitivity needed for COVID-19 detection and significantly reduce the turn-around time of reporting COVID-19 test results. Twit Text FOAVip Extractionless nucleic acid detection: a high capacity solution to COVID-19 testing ????Diagn Microbiol Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=34274751 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34274751 #FOAvip English Wikipedia <ref>{{Cite pmid|34274751}}</ref> Extractionless nucleic acid detection: a high capacity solution to COVID-19 testing #MMPMID34274751 Baksh S; Volodko N; Soucie M; Geier SB; Diep A; Rozak K; Chan TY; Mustapha J; Lai R; Estey M; Verity B; Lee MC Diagn Microbiol Infect Dis 2021[Oct]; 101 (2): 115458 PMID34274751show ga We describe an extractionless real-time reverse transcriptase-PCR (rRT-PCR) protocol for SARS-CoV-2 nucleic acid detection using heat as an accurate cost-effective high-capacity solution to COVID-19 testing. We present the effect of temperature, transport media, rRT-PCR mastermixes and gene assays on SARS-CoV-2 gene amplification and limits of detection. Utilizing our heated methodology, our limits of detection were 12.5 and 1 genome copy/reaction for singleplex E- and N1-gene assays, respectively, and 1 genome copy/reaction by utilizing an E/N1 or Orf1ab/N1 multiplex assay combination. Using this approach, we detected up to 98% of COVID-19 positive patient samples analyzed in our various cohorts including a significant percentage of weak positives. Importantly, this extractionless approach will allow for >2-fold increase in testing capacity with existing instruments, circumvent the additional need for expensive extraction devices, provide the sensitivity needed for COVID-19 detection and significantly reduce the turn-around time of reporting COVID-19 test results. |COVID-19 Nucleic Acid Testing/*methods/standards[MESH] |COVID-19/*diagnosis[MESH] |Fluorescence[MESH] |Hot Temperature[MESH] |Humans[MESH] |Multiplex Polymerase Chain Reaction[MESH] |SARS-CoV-2/genetics/*isolation & purification[MESH] |Sensitivity and Specificity[MESH] |Specimen Handling[MESH]Extractionless nucleic acid detection: a high capacity solution to COV(epmc).pdf DeepDyve Pubget Overpricing