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10.1016/j.ijid.2021.07.014 http://scihub22266oqcxt.onion/10.1016/j.ijid.2021.07.014 34273511!8278831!34273511     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34273511&cmd=llinks): Failed to open stream: HTTP request failed! 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Analysis of SARS-CoV-2 reverse transcription-quantitative polymerase chain reaction cycle threshold values vis-a-vis anti-SARS-CoV-2 antibodies from a high incidence region |pdf=|usr=}}{{34273511}} gab.com Text Analysis of SARS-CoV-2 reverse transcription-quantitative polymerase chain reaction cycle threshold values vis-a-vis anti-SARS-CoV-2 antibodies from a high incidence region JO: Int J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=34273511 #FOAvip OBJECTIVES: To examine the relationship between antibody status and cycle threshold (Ct) values, the prognostic value of the latter for COVID-19 patients, and the inter-assay comparability of SARS-CoV-2 Ct values. METHODS: In 347 COVID-19 inpatients, SARS-CoV-2 Ct values (via reverse transcription-quantitative polymerase chain reaction) on admission were compared between 2 assays and correlated with the antibody response (in the course of the disease), the clinical course and the time since onset of symptoms. RESULTS: Ct values for 2 of 3 target genes showed significant differences between the 2 assays used (P=0.012 and P<0.0001). Ct values were significantly higher for antibody positive patients (P<0.0001) and positively correlated with the amount of time since onset of symptoms (R: 0.332-0.363; P<0.001). Patients with fatal outcomes showed higher viral loads than survivors (P<0.0001). CONCLUSIONS: Ct values depend strongly on assay used and target gene examined and should not be used as quantitative values to guide therapeutic or diagnostic decisions. The inverse association between antibody status and viral load suggests that antibodies contribute to the elimination of the virus, independent of the outcome, which is influenced by the viral load on admission and might depend more strongly on other parts of the immune response. Twit Text FOAVip Analysis of SARS-CoV-2 reverse transcription-quantitative polymerase chain reaction cycle threshold values vis-a-vis anti-SARS-CoV-2 antibodies from a high incidence region ????Int J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=34273511 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34273511 #FOAvip English Wikipedia <ref>{{Cite pmid|34273511}}</ref> Analysis of SARS-CoV-2 reverse transcription-quantitative polymerase chain reaction cycle threshold values vis-a-vis anti-SARS-CoV-2 antibodies from a high incidence region #MMPMID34273511 Markewitz R; Torge A; Wandinger KP; Pauli D; Dargvainiene J; Franke A; Bujanda L; Marimon JM; Banales JM; Gutierrez-Stampa MA; Nafria B; Junker R Int J Infect Dis 2021[Sep]; 110 (ä): 114-122 PMID34273511show ga OBJECTIVES: To examine the relationship between antibody status and cycle threshold (Ct) values, the prognostic value of the latter for COVID-19 patients, and the inter-assay comparability of SARS-CoV-2 Ct values. METHODS: In 347 COVID-19 inpatients, SARS-CoV-2 Ct values (via reverse transcription-quantitative polymerase chain reaction) on admission were compared between 2 assays and correlated with the antibody response (in the course of the disease), the clinical course and the time since onset of symptoms. RESULTS: Ct values for 2 of 3 target genes showed significant differences between the 2 assays used (P=0.012 and P<0.0001). Ct values were significantly higher for antibody positive patients (P<0.0001) and positively correlated with the amount of time since onset of symptoms (R: 0.332-0.363; P<0.001). Patients with fatal outcomes showed higher viral loads than survivors (P<0.0001). CONCLUSIONS: Ct values depend strongly on assay used and target gene examined and should not be used as quantitative values to guide therapeutic or diagnostic decisions. The inverse association between antibody status and viral load suggests that antibodies contribute to the elimination of the virus, independent of the outcome, which is influenced by the viral load on admission and might depend more strongly on other parts of the immune response. |*COVID-19[MESH] |*SARS-CoV-2[MESH] |Humans[MESH] |Incidence[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |Reverse Transcription[MESH]Analysis of SARS-CoV-2 reverse transcription-quantitative polymerase c(epmc).pdf DeepDyve Pubget Overpricing