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10.1016/j.ymeth.2021.07.005 http://scihub22266oqcxt.onion/10.1016/j.ymeth.2021.07.005 34273478!8339599!34273478     free     free     free Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods 2022 ; 201 (ä): 26-33 Nephropedia Template TP {{tp|p=34273478|t=2022. Absolute quantification of SARS-CoV-2 with Clarity Plus digital PCR |pdf=|usr=}}{{34273478}} gab.com Text Absolute quantification of SARS-CoV-2 with Clarity Plus digital PCR JO: Methods http://www.kidney.de/pget.php?&tw=1&pmid=34273478 #FOAvip In recent years, the usage of digital polymerase chain reaction (dPCR) for various clinical applications has increased exponentially. In this study, a dPCR assay optimized on the Clarity Plus dPCR system was evaluated for the absolute quantification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the global coronavirus disease 2019 (COVID-19) outbreak. The assay demonstrated good inter- and intra- assay precision, accuracy, as well as excellent linearity across a range of over 6 orders of magnitude for target gene quantification. In addition, a comparison of the assay on both dPCR and qPCR platforms revealed that dPCR exhibited a slightly higher sensitivity compared to its qPCR counterpart when quantifying SARS-CoV-2 at a lower concentration. Overall, the results showed that the dPCR assay is a reliable and effective approach for the absolute quantification of SARS-CoV-2 and can be a valuable molecular tool in clinical applications such as detecting low viral loads in patients as well as in wastewater surveillance of COVID-19. Twit Text FOAVip Absolute quantification of SARS-CoV-2 with Clarity Plus digital PCR ????Methods http://www.kidney.de/pget.php?&tw=1&pmid=34273478 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34273478 #FOAvip English Wikipedia <ref>{{Cite pmid|34273478}}</ref> Absolute quantification of SARS-CoV-2 with Clarity Plus digital PCR #MMPMID34273478 Tan SYH; Kwek SYM; Low H; Pang YLJ Methods 2022[May]; 201 (ä): 26-33 PMID34273478show ga In recent years, the usage of digital polymerase chain reaction (dPCR) for various clinical applications has increased exponentially. In this study, a dPCR assay optimized on the Clarity Plus dPCR system was evaluated for the absolute quantification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the global coronavirus disease 2019 (COVID-19) outbreak. The assay demonstrated good inter- and intra- assay precision, accuracy, as well as excellent linearity across a range of over 6 orders of magnitude for target gene quantification. In addition, a comparison of the assay on both dPCR and qPCR platforms revealed that dPCR exhibited a slightly higher sensitivity compared to its qPCR counterpart when quantifying SARS-CoV-2 at a lower concentration. Overall, the results showed that the dPCR assay is a reliable and effective approach for the absolute quantification of SARS-CoV-2 and can be a valuable molecular tool in clinical applications such as detecting low viral loads in patients as well as in wastewater surveillance of COVID-19. |*COVID-19/diagnosis[MESH] |*SARS-CoV-2/genetics[MESH] |Humans[MESH] |RNA, Viral/analysis/genetics[MESH] |Real-Time Polymerase Chain Reaction[MESH] |Wastewater-Based Epidemiological Monitoring[MESH]Absolute quantification of SARS-CoV-2 with Clarity Plus digital PCR (epmc).pdf DeepDyve Pubget Overpricing