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10.1016/j.jviromet.2021.114241 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2021.114241 34273438!8279922!34273438     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2021 ; 296 (ä): 114241 Nephropedia Template TP {{tp|p=34273438|t=2021. SARS-CoV-2 saliva testing is a useful tool for Covid-19 diagnosis |pdf=|usr=}}{{34273438}} gab.com Text SARS-CoV-2 saliva testing is a useful tool for Covid-19 diagnosis JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=34273438 #FOAvip SARS-CoV-2 is the etiologic agent of coronavirus disease 2019 (COVID-19) and is mainly detected by RT-PCR methods from upper respiratory specimens, as recommended by the World Health Organization. Oro/nasopharyngeal swabbing can be discomfortable to the patients, requires trained healthcare personnel and may generate aerosol, increasing the risk of nosocomial infections. In this study, we describe two SARS-CoV-2 RNA extraction-free single RT-PCR protocols on saliva samples and compared the results with the paired oro/nasopharyngeal swab specimens from 400 patients. The two saliva protocols demonstrated a substantial agreement when compared to the oro/nasopharyngeal swab protocol. Moreover, the positivity rate of saliva protocols increased according to the disease period. The 95 % limit of detection of one of the therefore implemented saliva protocol was determined as 9441 copies/mL. Our results support the conclusion that RNA extraction-free RT-PCR using self-collected saliva specimens is an alternative to nasopharyngeal swabs, especially in the early phase of symptom onset. Twit Text FOAVip SARS-CoV-2 saliva testing is a useful tool for Covid-19 diagnosis ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=34273438 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34273438 #FOAvip English Wikipedia <ref>{{Cite pmid|34273438}}</ref> SARS-CoV-2 saliva testing is a useful tool for Covid-19 diagnosis #MMPMID34273438 de Oliveira CM; Brochi L; Scarpelli LC; Lopes ACW; Levi JE J Virol Methods 2021[Oct]; 296 (ä): 114241 PMID34273438show ga SARS-CoV-2 is the etiologic agent of coronavirus disease 2019 (COVID-19) and is mainly detected by RT-PCR methods from upper respiratory specimens, as recommended by the World Health Organization. Oro/nasopharyngeal swabbing can be discomfortable to the patients, requires trained healthcare personnel and may generate aerosol, increasing the risk of nosocomial infections. In this study, we describe two SARS-CoV-2 RNA extraction-free single RT-PCR protocols on saliva samples and compared the results with the paired oro/nasopharyngeal swab specimens from 400 patients. The two saliva protocols demonstrated a substantial agreement when compared to the oro/nasopharyngeal swab protocol. Moreover, the positivity rate of saliva protocols increased according to the disease period. The 95 % limit of detection of one of the therefore implemented saliva protocol was determined as 9441 copies/mL. Our results support the conclusion that RNA extraction-free RT-PCR using self-collected saliva specimens is an alternative to nasopharyngeal swabs, especially in the early phase of symptom onset. |COVID-19 Testing/*methods[MESH] |COVID-19/diagnosis/*virology[MESH] |Cross Infection/diagnosis[MESH] |Diagnostic Tests, Routine[MESH] |Health Personnel[MESH] |Humans[MESH] |Nasopharynx/virology[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2/genetics/*isolation & purification[MESH] |Saliva/*virology[MESH] |Specimen Handling/methods[MESH]SARS-CoV-2 saliva testing is a useful tool for Covid-19 diagnosis (epmc).pdf DeepDyve Pubget Overpricing