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10.1016/j.bios.2021.113486

http://scihub22266oqcxt.onion/10.1016/j.bios.2021.113486
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34260968!8264268!34260968
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suck abstract from ncbi


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pmid34260968      Biosens+Bioelectron 2021 ; 192 (ä): 113486
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  • A mask-based diagnostic platform for point-of-care screening of Covid-19 #MMPMID34260968
  • Daniels J; Wadekar S; DeCubellis K; Jackson GW; Chiu AS; Pagneux Q; Saada H; Engelmann I; Ogiez J; Loze-Warot D; Boukherroub R; Szunerits S
  • Biosens Bioelectron 2021[Nov]; 192 (ä): 113486 PMID34260968show ga
  • Diagnostics of SARS-CoV-2 infection using real-time reverse-transcription polymerase chain reaction (RT-PCR) on nasopharyngeal swabs is now well-established, with saliva-based testing being lately more widely implemented for being more adapted for self-testing approaches. In this study, we introduce a different concept based on exhaled breath condensate (EBC), readily collected by a mask-based sampling device, and detection with an electrochemical biosensor with a modular architecture that enables fast and specific detection and quantification of COVID-19. The face mask forms an exhaled breath vapor containment volume to hold the exhaled breath vapor in proximity to the EBC collector to enable a condensate-forming surface, cooled by a thermal mass, to coalesce the exhaled breath into a 200-500 muL fluid sample in 2 min. EBC RT-PCR for SARS-CoV-2 genes (E, ORF1ab) on samples collected from 7 SARS-CoV-2 positive and 7 SARS-CoV-2 negative patients were performed. The presence of SARS-CoV-2 could be detected in 5 out of 7 SARS-CoV-2 positive patients. Furthermore, the EBC samples were screened on an electrochemical aptamer biosensor, which detects SARS-CoV-2 viral particles down to 10 pfu mL(-1) in cultured SARS-CoV-2 suspensions. Using a "turn off" assay via ferrocenemethanol redox mediator, results about the infectivity state of the patient are obtained in 10 min.
  • |*Biosensing Techniques[MESH]
  • |*COVID-19[MESH]
  • |Exhalation[MESH]
  • |Humans[MESH]
  • |Point-of-Care Systems[MESH]
  • |RNA, Viral[MESH]


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