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10.1002/jmv.27204 http://scihub22266oqcxt.onion/10.1002/jmv.27204 34260086!8426709!34260086     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2021 ; 93 (12): 6575-6581 Nephropedia Template TP {{tp|p=34260086|t=2021. Effect of different storage conditions on COVID-19 RT-PCR results |pdf=|usr=}}{{34260086}} gab.com Text Effect of different storage conditions on COVID-19 RT-PCR results JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=34260086 #FOAvip Reliable and rapid detection of severe acute respiratory syndrome coronavirus 2 in laboratory setting is critical to control the pandemic. We aimed to an evaluated polymerase chain reaction (PCR) efficiency of nasopharyngeal swabs stored in viral transport medium (VTM) in different temperatures. Ninety swabs taken into VTM were analyzed at the first hour, then divided into two groups with similar numbers of positive and negative samples. Positive samples of each group were also subgrouped according to Fam CT values as low CT (<25), medium CT (25-32), and high CT (32-38) groups. One group was stored at 4 degrees C, while the other was stored at room temperature, PCR analyses were repeated every 24 h for 5 days and on Day 12. There was a total of 30 positive samples (12 low CT, 11 medium CT, and 7 high CT). The CT values of both groups remained unchanged in first 3 days while the CT values of the room temperature group increased after the third day. All of the positive samples remained positive in both groups for the first 5 days. On the 12th day, the total number of positives decreased to 8 in the room temperature group and 11 in the 4 degrees C groups. All the low CT samples remained positive in both groups. In conclusion, it is safe to store positive samples in room temperature for up to 5 days. Only samples with high viral loads remain positive for 12 days, regardless of whether stored at room temperature or 4 degrees C. Negative samples don't turn to invalid if stored in VTM. Twit Text FOAVip Effect of different storage conditions on COVID-19 RT-PCR results ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=34260086 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34260086 #FOAvip English Wikipedia <ref>{{Cite pmid|34260086}}</ref> Effect of different storage conditions on COVID-19 RT-PCR results #MMPMID34260086 Yilmaz Gulec E; Cesur NP; Yesilyurt Fazlioglu G; Kazezoglu C J Med Virol 2021[Dec]; 93 (12): 6575-6581 PMID34260086show ga Reliable and rapid detection of severe acute respiratory syndrome coronavirus 2 in laboratory setting is critical to control the pandemic. We aimed to an evaluated polymerase chain reaction (PCR) efficiency of nasopharyngeal swabs stored in viral transport medium (VTM) in different temperatures. Ninety swabs taken into VTM were analyzed at the first hour, then divided into two groups with similar numbers of positive and negative samples. Positive samples of each group were also subgrouped according to Fam CT values as low CT (<25), medium CT (25-32), and high CT (32-38) groups. One group was stored at 4 degrees C, while the other was stored at room temperature, PCR analyses were repeated every 24 h for 5 days and on Day 12. There was a total of 30 positive samples (12 low CT, 11 medium CT, and 7 high CT). The CT values of both groups remained unchanged in first 3 days while the CT values of the room temperature group increased after the third day. All of the positive samples remained positive in both groups for the first 5 days. On the 12th day, the total number of positives decreased to 8 in the room temperature group and 11 in the 4 degrees C groups. All the low CT samples remained positive in both groups. In conclusion, it is safe to store positive samples in room temperature for up to 5 days. Only samples with high viral loads remain positive for 12 days, regardless of whether stored at room temperature or 4 degrees C. Negative samples don't turn to invalid if stored in VTM. |COVID-19 Testing/*methods[MESH] |COVID-19/diagnosis[MESH] |Humans[MESH] |Real-Time Polymerase Chain Reaction/*methods/standards[MESH] |SARS-CoV-2[MESH] |Specimen Handling/*methods/standards[MESH]Effect of different storage conditions on COVID-19 RT-PCR results (epmc).pdf DeepDyve Pubget Overpricing