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10.1155/2021/6662027

http://scihub22266oqcxt.onion/10.1155/2021/6662027
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suck abstract from ncbi


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pmid34258278      Biomed+Res+Int 2021 ; 2021 (ä): 6662027
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  • Genome Features and In Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains #MMPMID34258278
  • Soloveva IV; Ilyicheva TN; Marchenko VY; Pyankov OV; Tochilina AG; Belova IV; Zhirnov VA; Bormotov NI; Skarnovich MO; Durymanov AG; Molodtsova SB; Filippova EI; Ovchinnikova AS; Magerramova AV; Ryzhikov AB; Maksyutov RA
  • Biomed Res Int 2021[]; 2021 (ä): 6662027 PMID34258278show ga
  • PURPOSE: The aim of this work was to analyze the complete genome of probiotic bacteria Lactobacillus plantarum 8 RA 3, Lactobacillus fermentum 90 TC-4, Lactobacillus fermentum 39, Bifidobacterium bifidum 791, Bifidobacterium bifidum 1, and Bifidobacterium longum 379 and to test their activity against influenza A and SARS-CoV-2 viruses. METHODS: To confirm the taxonomic affiliation of the bacterial strains, MALDI TOF mass spectrometry and biochemical test systems were used. Whole genome sequencing was performed on the Illumina Inc. MiSeq platform. To determine the antiviral activity, A/Lipetsk/1V/2018 (H1N1 pdm09) (EPI_ISL_332798) and A/common gull/Saratov/1676/2018 (H5N6) (EPI_ISL_336925) influenza viruses and SARS-CoV-2 virus strain Australia/VIC01/2020 (GenBank: MT007544.1) were used. RESULTS: All studied probiotic bacteria are nonpathogenic for humans and do not contain the determinants of transmission-type antibiotic resistance and integrated plasmids. Resistance to antibiotics of different classes is explained by the presence of molecular efflux pumps of the MatE and MFS families. Cultures of L. fermentum 90 TC 4, L. plantarum 8 RA 3, and B. bifidum 791 showed a pronounced activity against influenza A viruses in MDCK cells. Activity against the SARS-CoV-2 virus was demonstrated only by the L. fermentum 90 TC 4 strain in VERO cells. CONCLUSIONS: The studied probiotic bacteria are safe, have antiviral activity, and are of great importance for the prevention of diseases caused by respiratory viruses that can also infect the human intestine.
  • |Animals[MESH]
  • |Bifidobacterium longum/*genetics[MESH]
  • |COVID-19/*metabolism/therapy[MESH]
  • |Chlorocebus aethiops[MESH]
  • |Dogs[MESH]
  • |High-Throughput Nucleotide Sequencing[MESH]
  • |Humans[MESH]
  • |Influenza A Virus, H1N1 Subtype[MESH]
  • |Influenza, Human[MESH]
  • |Lactobacillus/*genetics[MESH]
  • |Madin Darby Canine Kidney Cells[MESH]
  • |Probiotics/*pharmacology[MESH]
  • |SARS-CoV-2/*metabolism[MESH]


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