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10.1007/s00705-021-05149-0 http://scihub22266oqcxt.onion/10.1007/s00705-021-05149-0 34251549!8273560!34251549     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Arch+Virol 2021 ; 166 (9): 2529-2540 Nephropedia Template TP {{tp|p=34251549|t=2021. ddPCR increases detection of SARS-CoV-2 RNA in patients with low viral loads |pdf=|usr=}}{{34251549}} gab.com Text ddPCR increases detection of SARS-CoV-2 RNA in patients with low viral loads JO: Arch Virol http://www.kidney.de/pget.php?&tw=1&pmid=34251549 #FOAvip RT-qPCR detection of SARS-CoV-2 RNA still represents the method of reference to diagnose and monitor COVID-19. From the onset of the pandemic, however, doubts have been expressed concerning the sensitivity of this molecular diagnosis method. Droplet digital PCR (ddPCR) is a third-generation PCR technique that is particularly adapted to detecting low-abundance targets. We developed two-color ddPCR assays for the detection of four different regions of SARS-CoV-2 RNA, including non-structural (IP4-RdRP, helicase) and structural (E, N) protein-encoding sequences. We observed that N or E subgenomic RNAs are generally more abundant than IP4 and helicase RNA sequences in cells infected in vitro, suggesting that detection of the N gene, coding for the most abundant subgenomic RNA of SARS-CoV-2, increases the sensitivity of detection during the highly replicative phase of infection. We investigated 208 nasopharyngeal swabs sampled in March-April 2020 in different hospitals of Greater Paris. We found that 8.6% of informative samples (n = 16/185, P < 0.0001) initially scored as "non-positive" (undetermined or negative) by RT-qPCR were positive for SARS-CoV-2 RNA by ddPCR. Our work confirms that the use of ddPCR modestly, but significantly, increases the proportion of upper airway samples testing positive in the framework of first-line diagnosis of a French population. Twit Text FOAVip ddPCR increases detection of SARS-CoV-2 RNA in patients with low viral loads ????Arch Virol http://www.kidney.de/pget.php?&tw=1&pmid=34251549 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34251549 #FOAvip English Wikipedia <ref>{{Cite pmid|34251549}}</ref> ddPCR increases detection of SARS-CoV-2 RNA in patients with low viral loads #MMPMID34251549 Marchio A; Batejat C; Vanhomwegen J; Feher M; Grassin Q; Chazal M; Raulin O; Farges-Berth A; Reibel F; Esteve V; Dejean A; Jouvenet N; Manuguerra JC; Pineau P Arch Virol 2021[Sep]; 166 (9): 2529-2540 PMID34251549show ga RT-qPCR detection of SARS-CoV-2 RNA still represents the method of reference to diagnose and monitor COVID-19. From the onset of the pandemic, however, doubts have been expressed concerning the sensitivity of this molecular diagnosis method. Droplet digital PCR (ddPCR) is a third-generation PCR technique that is particularly adapted to detecting low-abundance targets. We developed two-color ddPCR assays for the detection of four different regions of SARS-CoV-2 RNA, including non-structural (IP4-RdRP, helicase) and structural (E, N) protein-encoding sequences. We observed that N or E subgenomic RNAs are generally more abundant than IP4 and helicase RNA sequences in cells infected in vitro, suggesting that detection of the N gene, coding for the most abundant subgenomic RNA of SARS-CoV-2, increases the sensitivity of detection during the highly replicative phase of infection. We investigated 208 nasopharyngeal swabs sampled in March-April 2020 in different hospitals of Greater Paris. We found that 8.6% of informative samples (n = 16/185, P < 0.0001) initially scored as "non-positive" (undetermined or negative) by RT-qPCR were positive for SARS-CoV-2 RNA by ddPCR. Our work confirms that the use of ddPCR modestly, but significantly, increases the proportion of upper airway samples testing positive in the framework of first-line diagnosis of a French population. |COVID-19 Nucleic Acid Testing/instrumentation/*methods[MESH] |COVID-19/*diagnosis/epidemiology/virology[MESH] |Color[MESH] |Coronavirus Envelope Proteins/genetics[MESH] |Coronavirus Nucleocapsid Proteins/genetics[MESH] |France/epidemiology[MESH] |Gene Expression[MESH] |Humans[MESH] |Limit of Detection[MESH] |Nasopharynx/virology[MESH] |Phosphoproteins/genetics[MESH] |RNA Helicases/genetics[MESH] |RNA, Viral/*genetics[MESH] |RNA-Dependent RNA Polymerase/genetics[MESH] |SARS-CoV-2/*genetics[MESH] |Viral Load[MESH]ddPCR increases detection of SARS-CoV-2 RNA in patients with low viral(epmc).pdf DeepDyve Pubget Overpricing