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10.1073/pnas.2106724118 http://scihub22266oqcxt.onion/10.1073/pnas.2106724118 34244421!8325344!34244421     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proc+Natl+Acad+Sci+U+S+A 2021 ; 118 (30): ä Nephropedia Template TP {{tp|p=34244421|t=2021. Minute-scale detection of SARS-CoV-2 using a low-cost biosensor composed of pencil graphite electrodes |pdf=|usr=}}{{34244421}} gab.com Text Minute-scale detection of SARS-CoV-2 using a low-cost biosensor composed of pencil graphite electrodes JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=34244421 #FOAvip COVID-19 has led to over 3.47 million deaths worldwide and continues to devastate primarily middle- and low-income countries. High-frequency testing has been proposed as a potential solution to prevent outbreaks. However, current tests are not sufficiently low-cost, rapid, or scalable to enable broad COVID-19 testing. Here, we describe LEAD (Low-cost Electrochemical Advanced Diagnostic), a diagnostic test that detects severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) within 6.5 min and costs $1.50 per unit to produce using easily accessible and commercially available materials. LEAD is highly sensitive toward SARS-CoV-2 spike protein (limit of detection = 229 fg?mL(-1)) and displays an excellent performance profile using clinical saliva (100.0% sensitivity, 100.0% specificity, and 100.0% accuracy) and nasopharyngeal/oropharyngeal (88.7% sensitivity, 86.0% specificity, and 87.4% accuracy) samples. No cross-reactivity was detected with other coronavirus or influenza strains. Importantly, LEAD also successfully diagnosed the highly contagious SARS-CoV-2 B.1.1.7 UK variant. The device presents high reproducibility under all conditions tested and preserves its original sensitivity for 5 d when stored at 4 degrees C in phosphate-buffered saline. Our low-cost and do-it-yourself technology opens new avenues to facilitate high-frequency testing and access to much-needed diagnostic tests in resource-limited settings and low-income communities. Twit Text FOAVip Minute-scale detection of SARS-CoV-2 using a low-cost biosensor composed of pencil graphite electrodes ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=34244421 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34244421 #FOAvip English Wikipedia <ref>{{Cite pmid|34244421}}</ref> Minute-scale detection of SARS-CoV-2 using a low-cost biosensor composed of pencil graphite electrodes #MMPMID34244421 de Lima LF; Ferreira AL; Torres MDT; de Araujo WR; de la Fuente-Nunez C Proc Natl Acad Sci U S A 2021[Jul]; 118 (30): ä PMID34244421show ga COVID-19 has led to over 3.47 million deaths worldwide and continues to devastate primarily middle- and low-income countries. High-frequency testing has been proposed as a potential solution to prevent outbreaks. However, current tests are not sufficiently low-cost, rapid, or scalable to enable broad COVID-19 testing. Here, we describe LEAD (Low-cost Electrochemical Advanced Diagnostic), a diagnostic test that detects severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) within 6.5 min and costs $1.50 per unit to produce using easily accessible and commercially available materials. LEAD is highly sensitive toward SARS-CoV-2 spike protein (limit of detection = 229 fg?mL(-1)) and displays an excellent performance profile using clinical saliva (100.0% sensitivity, 100.0% specificity, and 100.0% accuracy) and nasopharyngeal/oropharyngeal (88.7% sensitivity, 86.0% specificity, and 87.4% accuracy) samples. No cross-reactivity was detected with other coronavirus or influenza strains. Importantly, LEAD also successfully diagnosed the highly contagious SARS-CoV-2 B.1.1.7 UK variant. The device presents high reproducibility under all conditions tested and preserves its original sensitivity for 5 d when stored at 4 degrees C in phosphate-buffered saline. Our low-cost and do-it-yourself technology opens new avenues to facilitate high-frequency testing and access to much-needed diagnostic tests in resource-limited settings and low-income communities. |*Biosensing Techniques[MESH] |*COVID-19 Testing[MESH] |*COVID-19/diagnosis/metabolism[MESH] |Electrodes[MESH] |Graphite/*chemistry[MESH] |Humans[MESH] |SARS-CoV-2/*metabolism[MESH] |Sensitivity and Specificity[MESH]Minute-scale detection of SARS-CoV-2 using a low-cost biosensor compos(epmc).pdf DeepDyve Pubget Overpricing