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10.1038/s41598-021-93604-y http://scihub22266oqcxt.onion/10.1038/s41598-021-93604-y 34239008!8266870!34239008     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 14149 Nephropedia Template TP {{tp|p=34239008|t=2021. Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains |pdf=|usr=}}{{34239008}} gab.com Text Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34239008 #FOAvip Cryopreservation of mouse spermatozoa is widely used for the efficient preservation and safe transport of valuable mouse strains. However, the current cryopreservation method requires special containers (plastic straws), undefined chemicals (e.g., skim milk), liquid nitrogen, and expertise when handling sperm suspensions. Here, we report an easy and quick (EQ) sperm freezing method. The main procedure consists of only one step: dissecting a single cauda epididymis in a microtube containing 20% raffinose solution, which is then stored in a -80 degrees C freezer. The frozen-thawed spermatozoa retain practical fertilization rates after 1 (51%) or even 3 months (25%) with the C57BL/6 J strain, the most sensitive strain for sperm freezing. More than half of the embryos thus obtained developed into offspring after embryo transfer. Importantly, spermatozoa stored at -80 degrees C can be transferred into liquid nitrogen for indefinite storage. As far as we know, our EQ method is the easiest and quickest method for mouse sperm freezing and should be applicable in all laboratories without expertise in sperm cryopreservation. This technique can help avoid the loss of irreplaceable strains because of closure of animal rooms in emergency situations such as unexpected microbiological contamination or social emergencies such as the COVID-19 threat. Twit Text FOAVip Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34239008 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34239008 #FOAvip English Wikipedia <ref>{{Cite pmid|34239008}}</ref> Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains #MMPMID34239008 Mochida K; Hasegawa A; Shikata D; Itami N; Hada M; Watanabe N; Tomishima T; Ogura A Sci Rep 2021[Jul]; 11 (1): 14149 PMID34239008show ga Cryopreservation of mouse spermatozoa is widely used for the efficient preservation and safe transport of valuable mouse strains. However, the current cryopreservation method requires special containers (plastic straws), undefined chemicals (e.g., skim milk), liquid nitrogen, and expertise when handling sperm suspensions. Here, we report an easy and quick (EQ) sperm freezing method. The main procedure consists of only one step: dissecting a single cauda epididymis in a microtube containing 20% raffinose solution, which is then stored in a -80 degrees C freezer. The frozen-thawed spermatozoa retain practical fertilization rates after 1 (51%) or even 3 months (25%) with the C57BL/6 J strain, the most sensitive strain for sperm freezing. More than half of the embryos thus obtained developed into offspring after embryo transfer. Importantly, spermatozoa stored at -80 degrees C can be transferred into liquid nitrogen for indefinite storage. As far as we know, our EQ method is the easiest and quickest method for mouse sperm freezing and should be applicable in all laboratories without expertise in sperm cryopreservation. This technique can help avoid the loss of irreplaceable strains because of closure of animal rooms in emergency situations such as unexpected microbiological contamination or social emergencies such as the COVID-19 threat. |Animals[MESH] |COVID-19[MESH] |Cryopreservation/instrumentation/*methods[MESH] |Embryo Transfer[MESH] |Emergencies[MESH] |Female[MESH] |Fertilization in Vitro/methods[MESH] |Male[MESH] |Mice[MESH] |Mice, Inbred C57BL[MESH]Easy and quick (EQ) sperm freezing method for urgent preservation of m(epmc).pdf DeepDyve Pubget Overpricing